PUBLICATION
Zebrafish YAC, BAC, and PAC genomic libraries
- Authors
- Amemiya, C.T., Zhong, T.P., Silverman, G.A., Fishman, M.C., and Zon, L.I.
- ID
- ZDB-PUB-990309-405
- Date
- 1999
- Source
- In The Zebrafish: Genetics and Genomics, H.W. Detrich, III, M. Westerfield, and L.I. Zon, eds., San Diego, CA: Academic Press, Methods Cell Biol. 60: 235-258 (Review)
- Registered Authors
- Amemiya, Chris, Fishman, Mark C., Zhong, Tao P., Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Animals
- Bacteria
- Chromosomes, Artificial, Yeast*
- Cloning, Molecular
- Genetic Testing/methods
- Genomic Library*
- Humans
- Internet
- Mice
- Zebrafish/genetics*
- PubMed
- 9891341 Full text @ In The Zebrafish: Genetics and Genomics, H.W. Detrich, III, M. Westerfield, and L.I. Zon, eds., San Diego, CA: Academic Press, Methods Cell Biol.
Citation
Amemiya, C.T., Zhong, T.P., Silverman, G.A., Fishman, M.C., and Zon, L.I. (1999) Zebrafish YAC, BAC, and PAC genomic libraries. In The Zebrafish: Genetics and Genomics, H.W. Detrich, III, M. Westerfield, and L.I. Zon, eds., San Diego, CA: Academic Press, Methods Cell Biol.. 60:235-258.
Abstract
Numerous positional cloning projects directed at isolating genes responsible for the myriads of observed developmental defects in the zebrafish are anticipated in the very near future. In this chapter, we have reviewed the YAC, BAC, and PAC large-insert genomic resources available to the zebrafish community. We have discussed how these resources are screened and used in a positional cloning scheme and have pointed out frequently formidable logistical considerations in the approach. Despite being extremely tedious, positional cloning projects in the zebrafish will be comparatively easier to accomplish than in human and mouse, because of unique biological advantages of the zebrafish system. Moreover, the ease and speed at which genes are identified and cloned should rapidly increase as more mapping reagents and information become available, thereby paving the way for meaningful biological studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping