PUBLICATION

Comparative synteny cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning

Authors
Karlstrom, R.O., Talbot, W.S., and Schier, A.F.
ID
ZDB-PUB-990302-44
Date
1999
Source
Genes & Development   13: 388-393 (Journal)
Registered Authors
Karlstrom, Rolf, Schier, Alexander, Talbot, William S.
Keywords
zebrafish mutations; Hh signaling; gli2; ventral forebrain
MeSH Terms
  • In Situ Hybridization
  • Embryonic Development
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Signal Transduction/genetics
  • RNA, Messenger/metabolism
  • Immunohistochemistry
  • Trans-Activators*
  • Transcription Factors/chemistry
  • Transcription Factors/genetics*
  • Chromosome Mapping
  • Genetic Linkage/genetics
  • Kruppel-Like Transcription Factors
  • Sequence Analysis, DNA
  • Cloning, Molecular
  • Mutation/genetics
  • Hedgehog Proteins
  • Animals
  • Prosencephalon/embryology
  • Prosencephalon/growth & development
  • Amino Acid Sequence
  • Zebrafish Proteins*
  • Proteins/genetics*
(all 23)
PubMed
10049354 Full text @ Genes & Dev.
Abstract
Zebrafish you-too (yot) mutations interfere with Hedgehog (Hh) signaling during embryogenesis. Using a comparative synteny approach, we isolated yot as a zinc finger transcription factor homologous to the Hh target gli2. Two alleles of yot contain nonsense mutations resulting in carboxy-terminally truncated proteins. In addition to causing defects in midline development, muscle differentiation, and retinal axon guidance, yot mutations disrupt anterior pituitary and ventral forebrain differentiation. yot mutations also cause ectopic lens formation in the ventral diencephalon. These findings reveal that truncated zebrafish Gli2 proteins interfere with Hh signaling necessary for differentiation and axon guidance in the ventral forebrain.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Karlstrom, R.O., Talbot, W.S., and Schier, A.F. (1999) Comparative synteny cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning. Genes & Development. 13:388-393.