PUBLICATION

Protein kinase A is involved in the induction of early mesodermal marker genes by activin

Authors
Joore, J., Van de Water, S., Betist, M., Van den Eijnden-van Raaij A., and Zivkovic, D.
ID
ZDB-PUB-990210-17
Date
1998
Source
Mechanisms of Development   79: 5-15 (Journal)
Registered Authors
Jongejan-Zivkovic, Dana, Joore, Jos
Keywords
zebrafish; Danio rerio; goosecoid; no tail; activin; protein kinase A; mesoderm
MeSH Terms
  • Bacterial Proteins*
  • Fetal Proteins/genetics
  • Fetal Proteins/metabolism
  • Goosecoid Protein
  • Enzyme Activation
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Mesoderm/drug effects
  • Mesoderm/metabolism*
  • Transcription Factors*
  • Zebrafish/embryology*
  • Zebrafish Proteins*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
  • Repressor Proteins*
  • Inhibins/metabolism*
  • Inhibins/pharmacology
  • Blastocyst/metabolism
  • Activins
  • Gene Expression Regulation, Developmental*
  • Animals
  • T-Box Domain Proteins*
  • Drosophila Proteins*
  • Cyclic AMP-Dependent Protein Kinases/metabolism*
  • Gastrula/physiology
  • Promoter Regions, Genetic
  • Signal Transduction
PubMed
10349616 Full text @ Mech. Dev.
Abstract
In this study we have investigated the role of cAMP-dependent protein kinase A (PKA) in the induction of the early mesodermal marker genes goosecoid and no tail by activin in zebrafish embryos. We show that upon treatment with activin, zebrafish blastula cells exhibit a rapid and transient increase in PKA activity. In these cells, activin rapidly induces the expression of the immediate early response genes goosecoid and no tail. Stimulation and inhibition of PKA by activin, respectively, enhances and reduces the induction of goosecoid and no tail mRNA expression. Similar effects of PKA stimulation and inhibition on the induction by activin of a 1.8 kb zebrafish goosecoid promoter construct were observed. The induction by activin of a fragment of the zebrafish goosecoid promoter that mediates an immediate early response to activin is blocked by inhibition of PKA. Activation of PKA alone has no effect in these experiments. Finally, inhibition of PKA in whole embryos by overexpression of a dominant negative regulatory subunit of PKA reduces the expression of no tail and goosecoid, whereas the expression of even-skippedl remains unaltered. Overexpression of the catalytic subunit of PKA in embryos does not affect expression of goosecoid, no tail or even-skippedl. These data show that in dissociated blastulae, PKA is required, but not sufficient for activin signalling towards induction of goosecoid and no tail. In intact zebrafish embryos, PKA contributes to induction of goosecoid and no tail, although it is not required or sufficient.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping