|ZFIN ID: ZDB-PUB-990112-1|
Cloning of zebrafish cDNA for 3ß-hydroxysteroid dehydrogenase and p450SCC
Lai, W.W.K., Hsiao, P.H., Guiguen, Y., and Chung, B.C.
|Source:||Endocrine research 24: 927-931 (Journal)|
|Registered Authors:||Chung, Bon-chu, Hsiao, Peihung, Lai, Wayne|
Lai, W.W.K., Hsiao, P.H., Guiguen, Y., and Chung, B.C. (1998) Cloning of zebrafish cDNA for 3ß-hydroxysteroid dehydrogenase and p450SCC. Endocrine research. 24:927-931.
ABSTRACTP450scc and 3beta-HSD cDNA were isolated from a zebrafish lambda gt10 cDNA library using trout SCC and 3beta-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3beta-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3beta-HSD and 53% similarities with the mouse 3beta-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3beta-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.
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