ZFIN ID: ZDB-PUB-990112-1
Cloning of zebrafish cDNA for 3ß-hydroxysteroid dehydrogenase and p450SCC
Lai, W.W.K., Hsiao, P.H., Guiguen, Y., and Chung, B.C.
Date: 1998
Source: Endocrine research   24: 927-931 (Journal)
Registered Authors: Chung, Bon-chu, Hsiao, Peihung, Lai, Wayne
Keywords: none
MeSH Terms:
  • 3-Hydroxysteroid Dehydrogenases/genetics*
  • Animals
  • Cholesterol Side-Chain Cleavage Enzyme/genetics*
  • Cloning, Molecular*
  • DNA, Complementary/genetics*
  • Humans
  • Isoenzymes/genetics
  • Oncorhynchus mykiss/genetics
  • Sequence Homology, Amino Acid
  • Zebrafish/genetics*
PubMed: 9888598
P450scc and 3beta-HSD cDNA were isolated from a zebrafish lambda gt10 cDNA library using trout SCC and 3beta-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3beta-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3beta-HSD and 53% similarities with the mouse 3beta-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3beta-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.