PUBLICATION
In vivo analysis using variants of zebrafish BMPR-IA: range of action and involvement of BMP in ectoderm patterning
- Authors
- Nikaido, M., Tada, M., Takeda, H., Kuroiwa, A., and Ueno, N.
- ID
- ZDB-PUB-990106-28
- Date
- 1999
- Source
- Development (Cambridge, England) 126: 181-190 (Journal)
- Registered Authors
- Nikaido, Masataka, Tada, Masazumi, Takeda, Hiroyuki, Ueno, Naoto
- Keywords
- zebrafish; BMP; BMP receptor; ectoderm patterning
- MeSH Terms
-
- Bone Morphogenetic Proteins/genetics
- Bone Morphogenetic Proteins/metabolism*
- Molecular Sequence Data
- Embryonic Induction/physiology
- Homeodomain Proteins*
- Trans-Activators/genetics
- Trans-Activators/metabolism
- Body Patterning/physiology*
- Sequence Homology, Amino Acid
- Zebrafish Proteins
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Neurons
- Receptors, Growth Factor/genetics
- Receptors, Growth Factor/metabolism*
- Signal Transduction
- Bone Morphogenetic Protein 2
- Animals
- Protein Serine-Threonine Kinases/genetics
- Protein Serine-Threonine Kinases/metabolism*
- Ectoderm/physiology*
- Transforming Growth Factor beta*
- Base Sequence
- Mesoderm
- Otx Transcription Factors
- Zebrafish/embryology*
- Zebrafish/genetics
- Bone Morphogenetic Protein Receptors, Type I
- Amino Acid Sequence
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Cloning, Molecular
- Embryo, Nonmammalian
- Xenopus laevis/genetics
- PubMed
- 9834197 Full text @ Development
Citation
Nikaido, M., Tada, M., Takeda, H., Kuroiwa, A., and Ueno, N. (1999) In vivo analysis using variants of zebrafish BMPR-IA: range of action and involvement of BMP in ectoderm patterning. Development (Cambridge, England). 126:181-190.
Abstract
It has been an intriguing problem whether the polypeptide growth factors belonging to the transforming growth factor-β (TGF-β) superfamily function as direct and long-range signaling molecules in pattern formation of the early embryo. In this study, we examined the mechanism of signal propagation of bone morphogenetic protein (BMP) in the ectodermal patterning of zebrafish embryos, in which BMP functions as an epidermal inducer and a neural inhibitor. To estimate the effective range of zbmp-2, we first performed whole-mount in situ hybridization analysis. The zbmp-2-expressing domain and the neuroectoderm, marked by otx-2 expression, were complementary, suggesting that BMP has a short-range effect in vivo. Moreover, mosaic experiments using a constitutively active form of a zebrafish BMP type I receptor (CA-BRIA) demonstrated that the cell-fate conversion, revealed by ectopic expression of gata-3 and repression of otx-2, occurred in a cell-autonomous manner, denying the involvement of the relay mechanism. We also found that zbmp-2 was induced cell autonomously within the transplanted cells in the host ectoderm, suggesting that BMP cannot influence even the neighboring cells. This result is consistent with the observation that there is no gap between the expression domains of zbmp-2 and otx-2. Taken together, we propose that, in ectodermal patterning, BMP exerts a direct and cell-autonomous effect to fate uncommitted ectodermal cells to become epidermis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping