PUBLICATION
ß-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos
- Authors
- Schneider, S., Steinbeisser, H., Warga, R.M., and Hausen, P.
- ID
- ZDB-PUB-981110-18
- Date
- 1996
- Source
- Mechanisms of Development 57: 191-198 (Journal)
- Registered Authors
- Warga, Rachel M.
- Keywords
- Xenopus; Zebrafish; Dorsalization; Nieuwkoop Center; Cortical rotation; beta-catenin; Nuclear localization; wnt-signaling
- MeSH Terms
-
- Animals
- Body Patterning/physiology*
- Cadherins/metabolism*
- Cell Nucleus/chemistry
- Cell Nucleus/metabolism
- Cytoskeletal Proteins/genetics*
- Cytoskeletal Proteins/metabolism
- Gene Expression Regulation, Developmental/physiology
- Lithium/pharmacology
- Trans-Activators*
- Xenopus/embryology*
- Xenopus Proteins
- Zebrafish/embryology*
- Zebrafish Proteins
- beta Catenin
- PubMed
- 8843396 Full text @ Mech. Dev.
Citation
Schneider, S., Steinbeisser, H., Warga, R.M., and Hausen, P. (1996) ß-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos. Mechanisms of Development. 57:191-198.
Abstract
The question of how dorsal-ventral polarity is established in vertebrates is central to our understanding of their early development. Several lines of evidence suggest that wnt-signaling is involved in the induction of dorsal-specific gene expression in the Spemann Organizer of amphibians. Here, we show that beta-catenin, acting as a component of the wnt-pathway, transiently accumulates in nuclei on the dorsal side of Xenopus and zebrafish blastulae. The spatially restricted nuclear translocation of beta-catenin precedes the expression of dorsal-specific genes. In experimentally ventralized frog embryos the dorsal ventral pattern of beta-catenin nuclear staining is abolished; in contrast, embryos hyperdorsalized by Li-ions or by injection of Xwnt8 mRNA exhibit an enhanced nuclear accumulation of beta-catenin. The results show that translocation of beta-catenin into nuclei in the wake of wnt-signaling is an early step in the establishment of the dorsal-ventral axis in frog and fish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping