|ZFIN ID: ZDB-PUB-981110-18|
ß-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos
Schneider, S., Steinbeisser, H., Warga, R.M., and Hausen, P.
|Source:||Mechanisms of Development 57: 191-198 (Journal)|
|Registered Authors:||Warga, Rachel M.|
|Keywords:||Xenopus; Zebrafish; Dorsalization; Nieuwkoop Center; Cortical rotation; beta-catenin; Nuclear localization; wnt-signaling|
|PubMed:||8843396 Full text @ Mech. Dev.|
Schneider, S., Steinbeisser, H., Warga, R.M., and Hausen, P. (1996) ß-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos. Mechanisms of Development. 57:191-198.
ABSTRACTThe question of how dorsal-ventral polarity is established in vertebrates is central to our understanding of their early development. Several lines of evidence suggest that wnt-signaling is involved in the induction of dorsal-specific gene expression in the Spemann Organizer of amphibians. Here, we show that beta-catenin, acting as a component of the wnt-pathway, transiently accumulates in nuclei on the dorsal side of Xenopus and zebrafish blastulae. The spatially restricted nuclear translocation of beta-catenin precedes the expression of dorsal-specific genes. In experimentally ventralized frog embryos the dorsal ventral pattern of beta-catenin nuclear staining is abolished; in contrast, embryos hyperdorsalized by Li-ions or by injection of Xwnt8 mRNA exhibit an enhanced nuclear accumulation of beta-catenin. The results show that translocation of beta-catenin into nuclei in the wake of wnt-signaling is an early step in the establishment of the dorsal-ventral axis in frog and fish embryos.
ADDITIONAL INFORMATION No data available