ZFIN ID: ZDB-PUB-981110-18
ß-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos
Schneider, S., Steinbeisser, H., Warga, R.M., and Hausen, P.
Date: 1996
Source: Mechanisms of Development   57: 191-198 (Journal)
Registered Authors: Warga, Rachel M.
Keywords: Xenopus; Zebrafish; Dorsalization; Nieuwkoop Center; Cortical rotation; beta-catenin; Nuclear localization; wnt-signaling
MeSH Terms:
  • Animals
  • Body Patterning/physiology*
  • Cadherins/metabolism*
  • Cell Nucleus/chemistry
  • Cell Nucleus/metabolism
  • Cytoskeletal Proteins/genetics*
  • Cytoskeletal Proteins/metabolism
  • Gene Expression Regulation, Developmental/physiology
  • Lithium/pharmacology
  • Trans-Activators*
  • Xenopus/embryology*
  • Xenopus Proteins
  • Zebrafish/embryology*
  • Zebrafish Proteins
  • beta Catenin
PubMed: 8843396 Full text @ Mech. Dev.
The question of how dorsal-ventral polarity is established in vertebrates is central to our understanding of their early development. Several lines of evidence suggest that wnt-signaling is involved in the induction of dorsal-specific gene expression in the Spemann Organizer of amphibians. Here, we show that beta-catenin, acting as a component of the wnt-pathway, transiently accumulates in nuclei on the dorsal side of Xenopus and zebrafish blastulae. The spatially restricted nuclear translocation of beta-catenin precedes the expression of dorsal-specific genes. In experimentally ventralized frog embryos the dorsal ventral pattern of beta-catenin nuclear staining is abolished; in contrast, embryos hyperdorsalized by Li-ions or by injection of Xwnt8 mRNA exhibit an enhanced nuclear accumulation of beta-catenin. The results show that translocation of beta-catenin into nuclei in the wake of wnt-signaling is an early step in the establishment of the dorsal-ventral axis in frog and fish embryos.