ZFIN ID: ZDB-PUB-981020-8
cyclops encodes a nodal-related factor involved in midline signaling
Rebagliati, M.R., Toyama, R., Haffter, P., Dawid, I.B.
Date: 1998
Source: Proceedings of the National Academy of Sciences of the United States of America   95: 9932-9937 (Journal)
Registered Authors: Dawid, Igor B., Haffter, Pascal, Rebagliati, Michael, Toyama, Reiko
Keywords: none
MeSH Terms:
  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Body Patterning/genetics*
  • Central Nervous System/embryology
  • DNA Primers/genetics
  • DNA, Complementary/genetics
  • Eye Abnormalities/embryology
  • Eye Abnormalities/genetics
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Mutation
  • Nodal Signaling Ligands
  • Phenotype
  • Proteins*
  • Signal Transduction/physiology
  • Transforming Growth Factor beta/genetics*
  • Transforming Growth Factor beta/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/physiology
  • Zebrafish Proteins*
PubMed: 9707578 Full text @ Proc. Natl. Acad. Sci. USA
Ventral structures in the central nervous system are patterned by signals emanating from the underlying mesoderm as well as originating within the neuroectoderm. Mutations in the zebrafish, Danio rerio, are proving instrumental in dissecting these midline signals. The cyclops mutation leads to a loss of medial floor plate and to severe deficits in ventral forebrain development, leading to cyclopia. Here, we report that the cyclops locus encodes the nodal-related protein Ndr2, a member of the transforming growth factor type beta superfamily of factors. The evidence includes identification of a missense mutation in the initiation codon and rescue of the cyclops phenotype by expression of ndr2 RNA, here renamed "cyclops." Thus, in interaction with other molecules implicated in these processes such as sonic hedgehog and one-eyed-pinhead, cyclops is required for ventral midline patterning of the embryonic central nervous system.