PUBLICATION

Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry

Authors
Rebagliati, M.R., Toyama, R., Fricke, C., Haffter, P., and Dawid, I.B.
ID
ZDB-PUB-980903-5
Date
1998
Source
Developmental Biology   199: 261-272 (Journal)
Registered Authors
Dawid, Igor B., Fricke, Cornelia, Haffter, Pascal, Rebagliati, Michael, Toyama, Reiko
Keywords
Danio rerio; zebrafish; nodal-related; forebrain; mesoderm; left-right asymmetry; organizer
MeSH Terms
  • In Situ Hybridization
  • Nodal Protein
  • Amino Acid Sequence
  • Female
  • Intracellular Signaling Peptides and Proteins
  • Xenopus Proteins*
  • Embryonic Induction/genetics
  • Embryonic Induction/physiology
  • Cloning, Molecular
  • Proteins/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Male
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Molecular Sequence Data
  • Nodal Signaling Ligands
  • Body Patterning/genetics*
  • Body Patterning/physiology
  • Species Specificity
  • DNA Primers/genetics
  • Xenopus/genetics
  • Transforming Growth Factor beta/genetics*
  • Transforming Growth Factor beta/physiology
  • Mice
  • Zebrafish Proteins*
  • Base Sequence
  • Animals
  • RNA/genetics
  • Gene Expression Regulation, Developmental
(all 30)
PubMed
9698446 Full text @ Dev. Biol.
Abstract
Nodal-related 1 (ndr1) and nodal-related 2 (ndr2) genes in zebrafish encode members of the nodal subgroup of the transforming growth factor-beta superfamily. We report the expression patterns and functional characteristics of these factors, implicating them in the establishment of dorsal-ventral polarity and left-right asymmetry. Ndr1 is expressed maternally, and ndr1 and ndr2 are expressed during blastula stage in the blastoderm margin. During gastrulation, ndr expression subdivides the shield into two domains: a small group of noninvoluting cells, the dorsal forerunner cells, express ndr1, while ndr2 RNA is found in the hypoblast layer of the shield and later in notochord, prechordal plate, and overlying anterior neurectoderm. During somitogenesis, ndr2 is expressed asymmetrically in the lateral plate as are nodal-related genes of other organisms, and in a small domain in the left diencephalon, providing the first observation of asymmetric gene expression in the embryonic forebrain. RNA injections into Xenopus animal caps showed that Ndr1 acts as a mesoderm inducer, whereas Ndr2 is an efficient neural but very inefficient mesoderm inducer. We suggest that Ndr1 has a role in mesoderm induction, while Ndr2 is involved in subsequent specification and patterning of the nervous system and establishment of laterality.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b160
    		Indel
    c4
      		Deficiency
      n1
        		Small Deletion
        ta72a
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          bmp2bta72a/ta72a (TU)
          Df(Chr15:chrd)c4/c4
          noton1/n1 (AB)
          tbxtab160/b160 (AB)
          WT
          1 - 5 of 5
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          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          No data available
          Mapping
          No data available
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          Citation
          Rebagliati, M.R., Toyama, R., Fricke, C., Haffter, P., and Dawid, I.B. (1998) Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry. Developmental Biology. 199:261-272.