PUBLICATION
Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm
- Authors
- Yamamoto, A., Amacher, S.L., Kim, S.-H., Geissert, D., Kimmel, C.B., and De Robertis, E.M.
- ID
- ZDB-PUB-980820-2
- Date
- 1998
- Source
- Development (Cambridge, England) 125: 3389-3397 (Journal)
- Registered Authors
- Amacher, Sharon, De Robertis, Eddy, Kimmel, Charles B.
- Keywords
- protocadherin; spadetail; floating head; gastrulation; convergence movements; somitogenesis; zebrafish
- MeSH Terms
-
- Body Patterning/genetics
- Gene Expression Regulation, Developmental
- DNA Primers/genetics
- Gastrula/cytology
- Cadherins/genetics*
- Cadherins/physiology
- Animals
- Sequence Homology, Amino Acid
- In Situ Hybridization
- Cloning, Molecular
- Base Sequence
- Transcription Factors/genetics*
- Transcription Factors/physiology
- Mesoderm/cytology
- Amino Acid Sequence
- Morphogenesis/genetics
- Cell Differentiation/genetics
- Models, Genetic
- Genes, Homeobox
- Molecular Sequence Data
- Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/physiology
- Somites/cytology
- PubMed
- 9693142 Full text @ Development
Citation
Yamamoto, A., Amacher, S.L., Kim, S.-H., Geissert, D., Kimmel, C.B., and De Robertis, E.M. (1998) Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm. Development (Cambridge, England). 125:3389-3397.
Abstract
Zebrafish paraxial protocadherin (papc) encodes a transmembrane cell adhesion molecule (PAPC) expressed in trunk mesoderm undergoing morphogenesis. Microinjection studies with a dominant-negative secreted construct suggest that papc is required for proper dorsal convergence movement s during gastrulation. Genetic studies show that papc is a close downstream target of spadetail, gene encoding a transcription factor required for mesodermal morphogenetic movements. Further, we show that the floating head homeobox gene is required in axial mesoderm to repress the expression of both spadetail and papc, promoting notochord and blocking differentiation of paraxial mesoderm. The PAPC structural cell-surface transcription factors and the actual cell biological behaviors that execute morphogenesis during gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping