PUBLICATION

The SCL gene specifies haemangioblast development from early mesoderm

Authors
Gering, M., Rodaway, A.R.F., Göttgens, B., Patient, R.K., and Green, A.R.
ID
ZDB-PUB-980820-1
Date
1998
Source
The EMBO journal   17: 4029-4045 (Journal)
Registered Authors
Gering, Martin, Patient, Roger K., Rodaway, Adam
Keywords
haemangioblast; haematopoiesis; SCL; vasculogenesis; zebrafish
MeSH Terms
  • Phylogeny
  • Sequence Analysis, DNA
  • Zebrafish Proteins*
  • Cell Differentiation
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/physiology
  • Mesoderm*/chemistry
  • Mesoderm*/cytology
  • Nuclear Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • MyoD Protein/genetics
  • Kidney Tubules/embryology
  • RNA, Messenger/analysis
  • Animals
  • Gene Expression Regulation, Developmental/physiology
  • Genes, Regulator/physiology*
  • Transcription Factors/genetics
  • Proto-Oncogene Proteins*
  • Basic Helix-Loop-Helix Transcription Factors
  • Hematopoietic Stem Cells/cytology*
  • Neovascularization, Physiologic
  • Somites
  • Molecular Sequence Data
  • PAX2 Transcription Factor
  • Amino Acid Sequence
  • Endothelium, Vascular/embryology
  • Cloning, Molecular
(all 28)
PubMed
9670018 Full text @ EMBO J.
Abstract
The SCL gene encodes a basic helix-loop-helix (bHLH) transcription factor that is essential for the development of all haematopoietic lineages. SCL is also expressed in endothelial cells, but its function is not essential for specification of endothelial progenitors and the role of SCL in endothelial development is obscure. We isolated the zebrafish SCL homologue and show that it was co-expressed in early mesoderm with markers of haematopoietic, endothelial and pronephric progenitors. Ectopic expression of SCL mRNA in zebrafish embryos resulted in overproduction of common haematopoietic and endothelial precursors, perturbation of vasculogenesis and concomitant loss of pronephric duct and somitic tissue. Notochord and neural tube formation were unaffected. These results provide the first evidence that SCL specifies formation of haemangioblasts, the proposed common precursor of blood and endothelial lineages. Our data also underline the striking similarities between the role of SCL in haematopoiesis/vasculogenesis and the function of other bHLH proteins in muscle and neural development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping