PUBLICATION
Pathfinding of olfactory neuron axons to stereotyped glomerular targets revealed by dynamic imaging in living zebrafish embryos
- Authors
- Dynes, J.L. and Ngai, J.
- ID
- ZDB-PUB-980729-35
- Date
- 1998
- Source
- Neuron 20: 1081-1091 (Journal)
- Registered Authors
- Dynes, Joseph, Ngai, John
- Keywords
- none
- MeSH Terms
-
- Neurites/chemistry
- Neurites/physiology*
- Plasmids
- Microscopy, Video
- Neuronal Plasticity/physiology
- Olfactory Receptor Neurons/cytology*
- Olfactory Receptor Neurons/physiology
- Olfactory Receptor Neurons/ultrastructure
- Afferent Pathways/cytology
- Afferent Pathways/embryology
- Afferent Pathways/physiology
- Boron Compounds
- Carbocyanines
- Fluorescent Dyes
- Luminescent Proteins
- Olfactory Bulb/cytology*
- Olfactory Bulb/embryology*
- Olfactory Bulb/physiology
- Animals
- Axons/physiology
- Animals, Genetically Modified
- Green Fluorescent Proteins
- Genes, Reporter
- Zebrafish
- PubMed
- 9655497 Full text @ Neuron
Citation
Dynes, J.L. and Ngai, J. (1998) Pathfinding of olfactory neuron axons to stereotyped glomerular targets revealed by dynamic imaging in living zebrafish embryos. Neuron. 20:1081-1091.
Abstract
In the vertebrate olfactory system, sensory neurons with common odorant specificities project to specific glomeruli in the olfactory bulb. How do olfactory sensory neurons find their glomerular targets? To address this question, we have visualized the genesis of the peripheral olfactory system in living zebrafish embryos. Dye labelings reveal that a primordial yet stereotyped map of glomeruli is apparent during embryogenesis. By labeling a small number of cells with an ectopically expressed green fluorescent protein reporter, we can observe the dynamic growth behaviors of individual olfactory neuron growth cones as they project to their glomeruli. We find that olfactory axons extend directly to their partner glomeruli, suggesting that these cells' growth cones rely upon pathfinding cues to reach their targets.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping