PUBLICATION

Promoting notochord fate and repressing muscle development in zebrafish axial mesoderm

Authors
Amacher, S.L. and Kimmel, C.B.
ID
ZDB-PUB-980601-13
Date
1998
Source
Development (Cambridge, England)   125: 1397-1406 (Journal)
Registered Authors
Amacher, Sharon, Kimmel, Charles B.
Keywords
floating head; spadetail; Xnot; not genes; no tail; Brachyury; genetic mosaic; floor plate; myogenesis
MeSH Terms
  • Embryo, Nonmammalian/physiology*
  • Gastrula/physiology*
  • Notochord/physiology*
  • Fetal Tissue Transplantation/physiology
  • Body Patterning/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Mesoderm/physiology*
  • Mutation
  • Models, Genetic
  • Embryonic Induction/genetics
  • Polymerase Chain Reaction
  • Nervous System/embryology*
  • Muscle, Skeletal/embryology*
  • Animals
(all 15)
PubMed
9502721 Full text @ Development
Abstract
Cell fate decisions in early embryonic cells are controlled by interactions among developmental regulatory genes. Zebrafish floating head mutants lack a notochord; instead, muscle forms under the neural tube. As shown previously, axial mesoderm in floating head mutant gastrulae fails to maintain expression of notochord genes and instead expresses muscle genes. Zebrafish spadetail mutant gastrulae have a nearly opposite phenotype; notochord markers are expressed in a wider domain than in wild-type embryos and muscle marker expression is absent. We examined whether these two phenotypes revealed an antagonistic genetic interaction by constructing the double mutant. Muscle does not form in the spadetail;floating head double mutant midline, indicating that spadetail function is required for floating head mutant axial mesoderm to transfate to muscle. Instead, the midline of spadetail;floating head double mutants is greatly restored compared to that of floating head mutants; the floor plate is almost complete and an anterior notochord develops. In addition, we find that floating head mutant cells can make both anterior and posterior notochord when transplanted into a wild-type host, showing that enviromental signals can override the predisposition of floating head mutant midline cells to make muscle. Taken together, these results suggest that repression of spadetail function by floating head is critical to promote notochord fate and prevent midline muscle development, and that cells can be recruited to the notochord by environmental signals.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
noton1/n1; tbx16b104/b104standard conditions5-9 somites
noton1/n1; tbx16b104/b104standard conditions5-9 somites
noton1/n1; tbx16b104/b104standard conditionsPrim-5
noton1/n1; tbx16b104/b104standard conditionsPrim-5
1 - 4 of 4
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    b333
      		Small Deletion
      n1
        		Small Deletion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        noton1/n1 (AB)
        noton1/n1; tbx16b104/b104
        slc25a37b333/b333
        tbx16b104/b104 (AB)
        1 - 4 of 4
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        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Amacher, S.L. and Kimmel, C.B. (1998) Promoting notochord fate and repressing muscle development in zebrafish axial mesoderm. Development (Cambridge, England). 125:1397-1406.