PUBLICATION
Progenitor cells in the adult zebrafish nervous system express a Brn-1-related POU gene, tai-ji
- Authors
- Huang, S. and Sato, S.
- ID
- ZDB-PUB-980415-6
- Date
- 1998
- Source
- Mechanisms of Development 71: 23-35 (Journal)
- Registered Authors
- Huang, Song, Sato, Sheryl M.
- Keywords
- tai-ji; zebrafish; Brn-1
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- Cell Line
- Gene Expression Regulation, Developmental*
- Genes, Homeobox/genetics*
- Homeodomain Proteins/biosynthesis
- Homeodomain Proteins/genetics
- Humans
- Nerve Tissue Proteins
- Nervous System/embryology
- Nervous System/metabolism*
- Neuropeptides/genetics*
- Organ Specificity/genetics
- POU Domain Factors
- Stem Cells/metabolism*
- Trans-Activators/genetics*
- Transcription Factors/biosynthesis
- Transcription Factors/genetics
- Zebrafish/genetics*
- Zebrafish Proteins
- PubMed
- 9507055 Full text @ Mech. Dev.
Citation
Huang, S. and Sato, S. (1998) Progenitor cells in the adult zebrafish nervous system express a Brn-1-related POU gene, tai-ji. Mechanisms of Development. 71:23-35.
Abstract
The adult fish brain undergoes continuous neurogenesis and retains the capacity to regenerate. However, the cellular and molecular basis of this process is not well understood. We report on the cloning and characterization of a Brain-1-related, class III POU domain gene, tai-ji, in the developing and adult zebrafish, as well as in a human cell line, hNT2. During development, as differentiation occurs, the expression of tai-ji is downregulated in the notochord, muscle, nervous system and dorsal fin. Similarly, tai-ji is expressed in the human neuronal precursor cell, hNT2, but is downregulated upon differentiation with retinoic acid. In the adult zebrafish nervous system, tai-ji persists in germinal zones, including cells in the germinal zone of the retina, the basal cells of the olfactory epithelium and cells of the subependymal zones in the optic tectum and telencephalon. Subsets of the tai-ji-expressing cells in these regions incorporate BrdU. Most of the tai-ji-expressing cells within these regions of the zebrafish brain are not differentiated and do not express a marker for post-mitotic neurons, acetylated tubulin nor do they express a marker of glial cells, glial acidic fibrillary protein (GFAP). We propose that the majority of the tai-ji-expressing cells are neural stem or progenitor cell populations that may represent the cellular basis for continuous growth in the adult nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping