PUBLICATION
Segmental expression of Hoxb-1 is controlled by a highly conserved autoregulatory loop dependent upon exd/pbx
- Authors
- Pöpperl, H., Bienz, M., Studer, M., Chan, S., Aparicio, S., Brenner, S., Mann, R., and Krumlauf, R.
- ID
- ZDB-PUB-980317-4
- Date
- 1995
- Source
- Cell 81: 1031-1042 (Journal)
- Registered Authors
- Aparicio, Sam, Chan, Shu Jin, Krumlauf, Robb
- Keywords
- none
- MeSH Terms
-
- Conserved Sequence
- Embryo, Mammalian/cytology
- Embryo, Mammalian/physiology
- Animals
- Regulatory Sequences, Nucleic Acid*
- Sequence Homology, Nucleic Acid
- Mice, Transgenic
- Multigene Family*
- Vertebrates
- Restriction Mapping
- Enhancer Elements, Genetic
- Base Sequence
- Genes, Homeobox*
- Drosophila/embryology
- Drosophila/metabolism
- Embryo, Nonmammalian/metabolism
- Recombinant Proteins/biosynthesis
- Gene Expression*
- Consensus Sequence
- Mice
- Fishes
- Rhombencephalon/metabolism*
- Homeodomain Proteins/biosynthesis*
- Homeodomain Proteins/genetics
- Chickens
- Molecular Sequence Data
- PubMed
- 7600572 Full text @ Cell
Citation
Pöpperl, H., Bienz, M., Studer, M., Chan, S., Aparicio, S., Brenner, S., Mann, R., and Krumlauf, R. (1995) Segmental expression of Hoxb-1 is controlled by a highly conserved autoregulatory loop dependent upon exd/pbx. Cell. 81:1031-1042.
Abstract
Comparison of Hoxb-1 regulatory regions from different vertebrates identified three related sequence motifs critical for rhombomere 4 (r4) expression in the hindbrain. Functional analysis in transgenic mice and Drosophila embryos demonstrated that the conserved elements are involved in a positive autoregulatory loop dependent on labial (lab) family members. Binding of Hoxb-1 to these elements in vitro requires cofactors, and the motifs closely resemble the consensus binding site for pbx1, a homolog of the Drosophila extradenticle (exd) homoedomain protein. In vitro exd/pbx serves as a Hoxb-1 cofactor in cooperative binding and in Drosophila expression mediated by the r4 enhancer is dependent on both lab and exd. This provides in vivo and in vitro evidence that r4 expression involves direct autoregulation dependent on cooperative interactions of Hoxb-1 with exd/pbx proteins as cofactors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping