PUBLICATION

Genetic analysis of chromosomal rearrangements in the cyclops region of the zebrafish genome

Authors
Talbot, W.S., Egan, E.S., Gates, M.A., Walker, C., Ullmann, B., Neuhauss, S.C.F., Kimmel, C.B., and Postlethwait, J.H.
ID
ZDB-PUB-980212-3
Date
1998
Source
Genetics   148: 373-380 (Journal)
Registered Authors
Egan, Elizabeth S., Gates, Michael A., Kimmel, Charles B., Neuhauss, Stephan, Postlethwait, John H., Talbot, William S., Ullmann, Bonnie, Walker, Charline
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Chromosome Mapping
  • Gene Rearrangement/genetics*
  • Genetic Markers/genetics
  • Translocation, Genetic*
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
9475747
Abstract
Genetic screens in zebrafish have provided mutations in hundreds of genes with essential functions in the developing embryo. To investigate the possible uses of chromosomal rearrangements in the analysis of these mutations, we genetically characterized three gamma-ray induced alleles of cyclops (cyc), a gene required for development of midline structures. We show that cyc maps near one end of Linkage Group 12 (LG 12) and that this region is involved in a reciprocal translocation with LG 2 in one gamma-ray induced mutation, cyc(b213). The translocated segments together cover approximately 5% of the genetic map, and we show that this rearrangement is useful for mapping cloned genes that reside in the affected chromosomal regions. The other two alleles, cyc(b16) and cyc(b229), have deletions in the distal region of LG 12. Interestingly, both of these mutations suppress recombination between genetic markers in LG 12, including markers at a distance from the deletion. This observation raises the possibility that these deletions affect a site required for meiotic recombination on the LG 12 chromosome. The cyc(b16) and cyc(b229) mutations may be useful for balancing other lethal mutations located in the distal region of LG 12. These results show that chromosomal rearrangements can provide useful resources for mapping and genetic analyses in zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes