|ZFIN ID: ZDB-PUB-980209-1|
Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation
Zhang, J., Talbot, W.S., and Schier, A.F.
|Source:||Cell 92: 241-251 (Journal)|
|Registered Authors:||Schier, Alexander, Talbot, William S., Zhang, Jiaojiao|
|PubMed:||9458048 Full text @ Cell|
Zhang, J., Talbot, W.S., and Schier, A.F. (1998) Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation. Cell. 92:241-251.
ABSTRACTThe zebrafish one-eyed pinhead (oep) mutation disrupts embryonic development, resulting in cyclopia and defects in endoderm, prechordal plate, and ventral neuroectoderm formation. We report the molecular isolation of oep using a positional cloning approach. The oep gene encodes a novel EGF- related protein with similarity to the EGF-CFC proteins cripto, cryptic, and FRL-1. Wild-type oep protein contains a functional signal sequence and is membrane-associated. Following ubiquitous maternal and zygotic expression, highest levels of oep mRNA are found in the gastrula margin and in axial structures and forebrain. Widespread misexpression of both membrane-attached and secreted forms of oep rescues prechordal plate and forebrain development in mutant embryos but does not lead to the ectopic induction of these cell types in wild-type fish. These results establish an essential but permissive role for an EGF-related ligand during vertebrate gastrulation.