PUBLICATION
Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation
- Authors
- Zhang, J., Talbot, W.S., and Schier, A.F.
- ID
- ZDB-PUB-980209-1
- Date
- 1998
- Source
- Cell 92: 241-251 (Journal)
- Registered Authors
- Schier, Alexander, Talbot, William S., Zhang, Jiaojiao
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- COS Cells
- Cell Membrane/chemistry
- Central Nervous System/embryology
- Cloning, Molecular/methods*
- Endoderm
- Epidermal Growth Factor/genetics
- Gastrula/chemistry
- Gastrula/physiology*
- Gene Expression Regulation, Developmental
- Genes/genetics
- Homeodomain Proteins/analysis
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/physiology
- Ligands
- Molecular Sequence Data
- Mutation
- Protein Sorting Signals/genetics
- RNA, Messenger/analysis
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Transcription Factors/analysis
- Transcription Factors/genetics*
- Transcription Factors/physiology
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 9458048 Full text @ Cell
Citation
Zhang, J., Talbot, W.S., and Schier, A.F. (1998) Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation. Cell. 92:241-251.
Abstract
The zebrafish one-eyed pinhead (oep) mutation disrupts embryonic development, resulting in cyclopia and defects in endoderm, prechordal plate, and ventral neuroectoderm formation. We report the molecular isolation of oep using a positional cloning approach. The oep gene encodes a novel EGF- related protein with similarity to the EGF-CFC proteins cripto, cryptic, and FRL-1. Wild-type oep protein contains a functional signal sequence and is membrane-associated. Following ubiquitous maternal and zygotic expression, highest levels of oep mRNA are found in the gastrula margin and in axial structures and forebrain. Widespread misexpression of both membrane-attached and secreted forms of oep rescues prechordal plate and forebrain development in mutant embryos but does not lead to the ectopic induction of these cell types in wild-type fish. These results establish an essential but permissive role for an EGF-related ligand during vertebrate gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping