PUBLICATION

Cloning and characterization of two isoforms of the zebrafish thyrotroph embryonic factor (tef-alpha and tef-beta)

Authors
Xu, X.Z., Liu, L.H., Wong, K.C.Y., and Ge, R.W.
ID
ZDB-PUB-980123-6
Date
1998
Source
Biochim. Biophys. Acta Gene Struct. Exp.   1395: 13-20 (Journal)
Registered Authors
Liu, Lihui
Keywords
TEF; PAR subfamily; bZIP transcription factor; gene regulation; zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Cloning, Molecular
  • Conserved Sequence/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger/analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors/genetics*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed
9434145 Full text @ Biochim. Biophys. Acta Gene Struct. Exp.
Abstract
We have cloned and sequenced two cDNAs representing two isoforms of the zebrafish thyrotroph embryonic factor (TEF) gene products (tef alpha and beta), a member of the PAR subfamily of bZIP transcription factors. The two isoforms encode two potential proteins of 300 and 293 amino acids respectively. Sequence comparison analysis indicate that the zebrafish tefs show high homology to PAR family of transcription factors of other species in the PAR domain, DNA binding domain and leucine zipper domain. Expression analysis by Northern blot and RT-PCR indicates that tef alpha and tefbeta are expressed in adult fish and throughout the zebrafish embryonic development. In addition, the two tefs are expressed in some adult tissues, but not others.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping