PUBLICATION
The zebrafish thyroid hormone receptor alpha1 is expressed during early embryogenesis and can function in transcriptional repression
- Authors
- Essner, J.J., Breuer, J.J., Essner, R.D., Fahrenkrug, S.C., and Hackett, P.B., Jr.
- ID
- ZDB-PUB-980123-3
- Date
- 1997
- Source
- Differentiation; research in biological diversity 62: 107-117 (Journal)
- Registered Authors
- Essner, Jeffrey, Fahrenkrug, Scott C., Hackett, Perry B.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blastocyst/metabolism
- Blotting, Northern
- Cloning, Molecular
- Embryo, Nonmammalian/metabolism*
- Gastrula/metabolism
- Gene Expression Regulation, Developmental*
- Genes, Reporter
- Molecular Sequence Data
- Oogenesis/physiology
- RNA, Messenger/analysis
- Receptors, Retinoic Acid/metabolism
- Receptors, Thyroid Hormone/genetics*
- Receptors, Thyroid Hormone/physiology
- Repressor Proteins/genetics*
- Repressor Proteins/physiology
- Ribonucleases/metabolism
- Sequence Homology, Amino Acid
- Transcription, Genetic*
- Tretinoin/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 9447705 Full text @ Differentiation
Citation
Essner, J.J., Breuer, J.J., Essner, R.D., Fahrenkrug, S.C., and Hackett, P.B., Jr. (1997) The zebrafish thyroid hormone receptor alpha1 is expressed during early embryogenesis and can function in transcriptional repression. Differentiation; research in biological diversity. 62:107-117.
Abstract
Nuclear receptors are a large family of ligand dependent transcription factors which participate in many diverse processes during development. In this report, we describe the cloning of the zebrafish thyroid hormone receptor alpha 1 (TR alpha 1) gene, the cellular counterpart of the viral oncogene v-erbA. TR alpha 1 is expressed during oogenesis and maternally supplied to the embryo. TR alpha 1 is expressed again after the mid blastula transition. By examining the effects of increased expression of TR alpha 1 on expression of a reporter gene which responds to both TR alpha 1 and retinoic acid receptors (RARs), we show that the zebrafish TR alpha 1 can act as a repressor during early zebrafish development before thyroid hormone is present in the embryo. In addition, our data suggest that TR alpha 1 can repress retinoic acid (RA)-signaling during early development. We propose that TR alpha 1 functions during early development as a transcriptional repressor, similar to the constitutive repressor activity of its viral counterpart v-erbA, which regulates anterior-posterior (A/P) patterning by repressing RA-signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping