PUBLICATION
Equivalence in the genetic control of hindbrain segmentation in fish and mouse
- Authors
- Moens, C.B., Cordes, S.P., Giorgianni, M.W., Barsh, G.S., and Kimmel, C.B,
- ID
- ZDB-PUB-980120-2
- Date
- 1998
- Source
- Development (Cambridge, England) 125: 381-391 (Journal)
- Registered Authors
- Kimmel, Charles B., Moens, Cecilia
- Keywords
- hindbrain segmentation; zebrafish; valentino (val); kreisler; rhombomere
- MeSH Terms
-
- Molecular Sequence Data
- DNA-Binding Proteins/genetics
- Body Patterning/genetics*
- Genes/genetics
- Leucine Zippers
- Branchial Region/embryology
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/physiology
- Zebrafish
- Somites/chemistry
- Avian Proteins*
- Ear, Inner/embryology
- Zebrafish Proteins*
- RNA, Messenger/analysis
- Polymorphism, Restriction Fragment Length
- Time Factors
- Maf Transcription Factors
- Oncogene Proteins*
- Amino Acid Sequence
- MafB Transcription Factor
- Cloning, Molecular
- Gene Expression Regulation, Developmental/physiology*
- Mosaicism
- Rhombencephalon/embryology*
- Sequence Homology, Amino Acid
- Mutation
- Transcription Factors/genetics
- Animals
- Sequence Analysis, DNA
- Mice
- Chromosome Mapping
- PubMed
- 9425134 Full text @ Development
Citation
Moens, C.B., Cordes, S.P., Giorgianni, M.W., Barsh, G.S., and Kimmel, C.B, (1998) Equivalence in the genetic control of hindbrain segmentation in fish and mouse. Development (Cambridge, England). 125:381-391.
Abstract
The vertebrate hindbrain is subdivided into a series of rhombomeres whose segmental organization serves to pattern the architecture and innervation of the developing head. The zebrafish gene valentino is required cell-autonomously in the development of rhombomeres 5 and 6, and valentino mutants lack visible hindbrain segmentation caudal to the r3/4 boundary (Moens, C. B., Yan, Y.-L., Appel, B., Force, A. G., and Kimmel, C. B. (1996) Development 122, 3981-3990). Here we show that valentino is the zebrafish homologue of the mouse segmentation gene kreisler, which encodes a bZip transcription factor. The valentino gene is expressed in a manner consistent with its proposed role in subdividing rhombomeres 5 and 6 from their common precursor 'proto-segment' in the presumptive hindbrain, a process that we also demonstrate is reflected in the normal order of appearance of rhombomere boundaries. As well as having similar phenotypes with respect to visible hindbrain segmentation and patterns of marker gene expression, valentino and kreisler mutants have similar pharyngeal arch and inner ear defects, consistent with a conserved role for this gene in hindbrain segmentation and in patterning of the head periphery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping