|ZFIN ID: ZDB-PUB-971203-8|
A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula
Fürthauer, M., Thisse, C., and Thisse, B.
|Source:||Development (Cambridge, England) 124(21): 4253-4264 (Journal)|
|Registered Authors:||Fürthauer, Maximilian, Thisse, Bernard, Thisse, Christine|
|Keywords:||fgf-8; Spemann's organizer; gastrulation; dorsoventral patterning; convergence; BMP2; BMP4; Chordin; Noggin; Goosecoid|
Fürthauer, M., Thisse, C., and Thisse, B. (1997) A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula. Development (Cambridge, England). 124(21):4253-4264.
ABSTRACTSignals released from Spemann's organizer, together with ventralizing factors such as BMPs, are necessary to pattern the dorsoventral axis of the vertebrate embryo. We report that a member of the FGF family, fgf-8, not secreted by the axial mesoderm but expressed in a dorsoventral gradient at the margin of the zebrafish gastrula, also contributes to the establishment of the dorsoventral axis of the embryo. Ectopic expression of FGF-8 leads to the expansion of dorsolateral derivatives at the expense of ventral and posterior domains. Moreover, FGF-8 displays some organizer properties as it induces the formation of a partial secondary axis in the absence of factors released from Spemann's organizer territory. Analysis of its interaction with the ventralizing factors, BMPs, reveals that overexpression of FGF-8 inhibits the expression of these factors in the ventral part of the embryo as early as blastula stage, suggesting that FGF-8 acts upstream of BMP2 and BMP4. We conclude that FGF-8 is involved in defining dorsoventral identity and is an important organizing factor responsible for specification of mesodermal and ectodermal dorsolateral territories of the zebrafish gastrula.