ZFIN ID: ZDB-PUB-971203-6
Expression of c-ret in the zebrafish embryo: potential roles in motoneuronal development
Bisgrove, B.W., Raible, D.W., Walter, V., Eisen, J.S., and Grunwald, D.J.
Date: 1997
Source: Journal of neurobiology   33(6): 749-768 (Journal)
Registered Authors: Bisgrove, Brent, Eisen, Judith S., Grunwald, David, Raible, David
Keywords: c-ret; receptor tyrosine kinase; zebrafish; embryogenesis; motoneurons
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/physiology
  • Branchial Region/embryology
  • Branchial Region/physiology
  • DNA/isolation & purification
  • Drosophila Proteins*
  • Embryo, Nonmammalian/physiology
  • Enteric Nervous System/embryology
  • Enteric Nervous System/physiology
  • Gene Expression Regulation, Developmental*
  • Immunohistochemistry
  • Molecular Sequence Data
  • Motor Neurons/physiology*
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology*
  • Proto-Oncogene Proteins c-ret
  • RNA/isolation & purification
  • Receptor Protein-Tyrosine Kinases/genetics
  • Receptor Protein-Tyrosine Kinases/physiology*
  • Sequence Analysis, DNA
  • Spinal Cord/embryology
  • Spinal Cord/physiology
  • Zebrafish
  • Zebrafish Proteins
PubMed: 9369149 Full text @ J. Neurobiol.
We have isolated and characterized the zebrafish ortholog of c-ret, a gene essential for renal organogenesis and enteric nervous system development in mammals. During zebrafish embryogenesis c-ret transcripts are expressed in a number of tissues including spinal motoneurons, pronephric ducts, cranial ganglia, pharyngeal arches, and the enteric nervous system. We have examined in detail the expression of c-ret during the development of identified spinal primary motoneurons. c-ret expression is regulated in a cell type-specific manner among the three primary motoneurons. c-ret is expressed at its highest levels in caudal primary (CaP) motoneurons and transcripts can be detected shortly before the expression of the CaP-specific gene, islet2. We suggest that c-ret may play a role in specifying CaP cell identity. c-ret is expressed at low levels in the other primary motoneurons and also in a subset of secondary motoneurons, suggesting that it may also play a broader role in motoneuronal survival or maintenance.