PUBLICATION

GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene

Authors
Long, Q.M., Meng, A.M., Wang, H., Jessen, J.R., Farrell, M.J., and Lin, S.
ID
ZDB-PUB-971203-3
Date
1997
Source
Development (Cambridge, England)   124(20): 4105-4111 (Journal)
Registered Authors
Farrell, Michael, Jessen, Jason R., Lin, Shuo, Meng, Anming, Wang, Han
Keywords
transgenesis; hematopoiesis; zebrafish; GATA-1; green fluorescent protein; GFP
MeSH Terms
  • Animals
  • Green Fluorescent Proteins
  • Animals, Genetically Modified
  • Gene Expression Regulation, Developmental*
  • DNA-Binding Proteins/genetics*
  • Gene Transfer Techniques
  • Zebrafish/embryology*
  • Transcription Factors/genetics*
  • GATA1 Transcription Factor
  • Luminescent Proteins/genetics*
  • Erythroid-Specific DNA-Binding Factors
  • Zebrafish Proteins
(all 12)
PubMed
9374406 Full text @ Development
Abstract
In this study, DNA constructs containing the putative zebrafish promoter sequences of GATA-1, an erythroid-specific transcription factor, and the green fluorescent protein reporter gene, were microinjected into single-cell zebrafish embryos. Erythroid-specific activity of the GATA-1 promoter was observed in living embryos during early development. Fluorescent circulating blood cells were detected in microinjected embryos 24 hours after fertilization and were still present in 2-month-old fish. Germline transgenic fish obtained from the injected founders continued to express green fluorescent protein in erythroid cells in the F1 and F2 generations. The green fluorescent protein expression patterns in transgenic fish were consistent with the pattern of GATA-1 mRNA expression detected by RNA in situ hybridization. These transgenic fish have allowed us to isolate, by fluorescence-activated cell sorting, the earliest erythroid progenitor cells from developing embryos for in vitro studies. By generating transgenic fish using constructs containing other zebrafish promoters and green fluorescent protein reporter gene, it should be possible to visualize the origin and migration of any lineage-specific progenitor cells in a living embryo.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la781TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    la781Tg
    WT
    1 - 2 of 2
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Long, Q.M., Meng, A.M., Wang, H., Jessen, J.R., Farrell, M.J., and Lin, S. (1997) GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development (Cambridge, England). 124(20):4105-4111.