PUBLICATION
A clonal analysis of spinal cord development in the zebrafish
- Authors
- Papan, C. and Campos-Ortega, J.A.
- ID
- ZDB-PUB-970917-11
- Date
- 1997
- Source
- Development genes and evolution 207(2): 71-81 (Journal)
- Registered Authors
- Campos-Ortega, Jose, Papan, Cyrus
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
Papan, C. and Campos-Ortega, J.A. (1997) A clonal analysis of spinal cord development in the zebrafish. Development genes and evolution. 207(2):71-81.
Abstract
We describe the results of a clonal analysis of spinal cord development in the zebrafish. The data were obtained from embryos in which fluorescent lineage tracer was injected into single cells in the neural plate at the two-somite stage. Injected animals were allowed to survive until either 4 days or 2 weeks postfertilization. In other experiments, bromodeoxy uridine (BrdU) was injected intraperitoneally at 30 h postfertilization (hpf) after lineage tracer injection in the neural plate at the two-somite stage, and the embryos fixed at 38 hpf. We restricted our experiments to the thoracic region of the spinal cord. Our experiments were aimed at answering questions regarding the proliferative abilities of neuroepithelial cells during embryonic development (as deduced from the size of the clones), the modes of cell division (as deduced from the uptake of BrdU into clone cells), positional differences in the proliferation of cells within the neural plate itself, the cellular composition of the clones, and cell dispersion (deduced from the regional distribution of clone cells).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping