PUBLICATION

A novel zebrafish gene expressed specifically in the photoreceptor cells of the retina

Authors
Chang, H. and Gilbert, W.
ID
ZDB-PUB-970903-7
Date
1997
Source
Biochemical and Biophysical Research Communications   237(1): 84-89 (Journal)
Registered Authors
Chang, H., Gilbert, Walter
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins/chemistry
  • Basic Helix-Loop-Helix Transcription Factors
  • Blotting, Western
  • Cloning, Molecular
  • Escherichia coli/genetics
  • Escherichia coli Proteins*
  • Eye Proteins/biosynthesis*
  • Eye Proteins/chemistry*
  • Homeodomain Proteins*
  • Humans
  • Immunohistochemistry
  • Mitochondrial Proteins*
  • Molecular Sequence Data
  • Photoreceptor Cells/metabolism*
  • Proteins/chemistry
  • RNA, Messenger/biosynthesis
  • Recombinant Proteins/biosynthesis
  • Recombinant Proteins/chemistry
  • Recombinant Proteins/immunology
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed
9266834 Full text @ Biochem. Biophys. Res. Commun.
Abstract
We have identified and characterized a novel protein from adult zebrafish retina, which we named ES1. Database search revealed that the ES1 gene has significant similarity to two genes with unknown functions: the Escherichia coli sigma cross-reacting protein 27a (scrp27a) and the human KNP-I/GT335. In situ hybridization and immunohistochemistry experiments showed that both ES1 mRNA and protein are expressed specifically in adult photoreceptor cells. ES1 seems to be a cytoplasmic protein. An ES1-like antigen was also detected in photoreceptor cells of goldfish with anti-ES1 antibodies. The retina specific expression and the evolutionary conservation suggest that ES1 protein may be important for maintaining normal retina structure and function.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping