ZFIN ID: ZDB-PUB-970724-3
Expression of an L1-related cell adhesion molecule on developing CNS fiber tracts in zebrafish and its functional contribution to axon fasciculation
Weiland, U.M., Ott, H., Bastmeyer, M., Schaden, H., Giordano, S. and Stürmer, C.A.O.
Date: 1997
Source: Molecular and cellular neurosciences   9(1): 77-89 (Journal)
Registered Authors: Bastmeyer, Martin, Giordano, Suzanne, Ott, Heiko, Stuermer, Claudia, Weiland, U.M.
Keywords: none
MeSH Terms:
  • Animals
  • Axons/physiology*
  • Cell Adhesion Molecules, Neuronal/metabolism*
  • Central Nervous System/growth & development*
  • Gene Expression Regulation, Developmental
  • Immunohistochemistry
  • Zebrafish
PubMed: 9204481 Full text @ Mol. Cell Neurosci.
ABSTRACT
E587 antigen, an L1-related cell adhesion molecule, is expressed by growing axons and has previously been shown to enhance axon growth and to mediate fasciculation of axons from newborn retinal ganglion cells in goldfish. In zebrafish, the monoclonal antibody E17 against E587 antigen stains all axons in the primary tracts and commissures from 17 h postfertilization (pf) onward and axons which are added subsequently to this scaffold. Moreover, Fab fragments of an E587 antiserum (E587 Fabs) injected into the ventricle of 30-h pf zebrafish embryos caused a marked defasciculation of distinct axon bundles in the posterior commissure, in hindbrain commissures, and in longitudinal tracts of the hindbrain, where they also caused increased crossings between fascicles. The regulated expression of E587 antigen by all developing axons and the effects caused by E587 Fabs show that E587 antigen contributes to the formation of tight and orderly fascicles in the developing CNS.
ADDITIONAL INFORMATION