PUBLICATION

Inactivation of the zebrafish homologue of Chx10 by antisense oligonucleotides causes eye malformations similar to the ocular retardation phenotype

Authors
Barabino, S.M.L., Spada, F., Cotelli, F., and Boncinelli, E.
ID
ZDB-PUB-970722-18
Date
1997
Source
Mechanisms of Development   63(2): 133-143 (Journal)
Registered Authors
Barabino, S.M.L., Cotelli, Franco
Keywords
antisense oligonucleotides; prd-type homeodomain; eye morphogenesis; zebrafish
MeSH Terms
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Oligonucleotides, Antisense/pharmacology
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Regulation, Developmental*
  • Eye Abnormalities/genetics
  • Eye Abnormalities/physiopathology*
  • Tissue Distribution
  • Zebrafish Proteins*
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/physiology*
  • Dose-Response Relationship, Drug
  • Animals
  • Eye Proteins/biosynthesis
  • Eye Proteins/genetics*
  • Amino Acid Sequence
  • In Situ Hybridization
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics*
  • Transcription Factors/physiology*
(all 23)
PubMed
9203137 Full text @ Mech. Dev.
Abstract
We report the cloning of a zebrafish paired-type homeobox gene, Alx, closely related to the murine Chx10 and the gold fish Vsx-I homeodomain proteins. Alx is first expressed at about 12 h post-fertilization (hpf) when optic vesicles appear. Its expression is restricted to the early retinal neuroepithelium, whereas no signal can be detected in the optic placode. Later, Alx expression follows the differentiation of the neural retina. Inhibition experiments with antisense oligonucleotides resulted in specific eye malformations which are reminiscent of the phenotype of ocular retardation (or) mice, caused by a spontaneous Chx10 mutation. The expression of other developmentally relevant genes such as pax(zf-a), pax(zf-b) and krx-20 was not affected in the antisense treated embryos.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Barabino, S.M.L., Spada, F., Cotelli, F., and Boncinelli, E. (1997) Inactivation of the zebrafish homologue of Chx10 by antisense oligonucleotides causes eye malformations similar to the ocular retardation phenotype. Mechanisms of Development. 63(2):133-143.