PUBLICATION
Isolation and characterization of a carbonic anhydrase homologue from the zebrafish (Danio rerio)
- Authors
- Peterson, R.E., Tu, C.K., and Linser, P.J.
- ID
- ZDB-PUB-970507-64
- Date
- 1997
- Source
- Journal of molecular evolution 44(4): 432-439 (Journal)
- Registered Authors
- Linser, Paul J., Peterson, Robert (Chuck)
- Keywords
- carbonic anhydrase; zebrafish; Danio rerio; retina; molecular evolution; teleost; cyprinid
- MeSH Terms
-
- Animals
- Evolution, Molecular
- Zebrafish/genetics*
- Molecular Sequence Data
- Cloning, Molecular
- Phylogeny*
- Kinetics
- DNA Primers
- Carbonic Anhydrases/genetics*
- Carbonic Anhydrases/isolation & purification
- Carbonic Anhydrases/metabolism
- Genetic Variation
- Base Sequence
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Amino Acid Sequence
- PubMed
- 9089083 Full text @ J. Mol. Evol.
Citation
Peterson, R.E., Tu, C.K., and Linser, P.J. (1997) Isolation and characterization of a carbonic anhydrase homologue from the zebrafish (Danio rerio). Journal of molecular evolution. 44(4):432-439.
Abstract
We have isolated a 29,000-Da carbonic anhydrase (CA) protein from the zebrafish, Danio rerio, sequenced two peptide fragments, and tentatively identified it as a high-activity CA by inhibition kinetics. We have also characterized a 1,537-bp message whose deduced sequence of 260 amino acids matches that of the isolated protein. This CA is clearly an alpha-CA based on the similarity of its sequence to that of other members of the alpha-CA gene family. A phylogenetic analysis suggested CAH-Z diverged after the branching of the CA-V and CA-VII genes and prior to the duplications that generated the CA-I, CA-II, and CA-III genes of amniotes. This marks the first characterization of the mRNA and its protein product from the CA gene of a teleost.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping