PUBLICATION
Specific craniofacial cartilage dysmorphogenesis coincides with a loss of dlx gene expression in retinoic acid-treated zebrafish embryos
- Authors
- Ellies, D.L., Langille, R.M., Martin, C.C., Akimenko, M.A., and Ekker, M.
- ID
- ZDB-PUB-970327-1
- Date
- 1997
- Source
- Mechanisms of Development 61(1-2): 23-36 (Journal)
- Registered Authors
- Akimenko, Marie-Andree, Ekker, Marc, Martin, C. Cristofre
- Keywords
- cartilage; head skeleton; homeobox genes; neural crest; retinoic acid; zebrafish
- MeSH Terms
-
- Tretinoin/pharmacology*
- Cell Differentiation
- Face/abnormalities*
- In Situ Hybridization
- Time Factors
- Apoptosis
- Neural Crest/cytology
- RNA, Messenger/genetics
- Transcription Factors*
- Homeodomain Proteins/physiology*
- Animals
- Genes, Homeobox*
- Gene Expression Regulation, Developmental/drug effects*
- Cartilage/embryology*
- Zebrafish/embryology*
- PubMed
- 9076675 Full text @ Mech. Dev.
Citation
Ellies, D.L., Langille, R.M., Martin, C.C., Akimenko, M.A., and Ekker, M. (1997) Specific craniofacial cartilage dysmorphogenesis coincides with a loss of dlx gene expression in retinoic acid-treated zebrafish embryos. Mechanisms of Development. 61(1-2):23-36.
Abstract
Treatments of zebrafish embryos with retinoic acid (RA), a substance known to cause abnormal craniofacial cartilage development in other vertebrates, result in dose- and stage- dependent losses of dlx homeobox gene expression in several regions of the embryo. Dlx expression in neural crest cells migrating from the hindbrain and in the visceral arch primordia is particularly sensitive to RA treatment. The strongest effects are observed when RA is administered prior to or during crest cell migration but effects can also be observed if RA is applied when the cells have entered the primordia of the arches. Losses of dlx expression correlate either with the loss of cartilage elements originating from hindbrain neural crest cells or with abnormal morphology of these elements. Cartilage elements that originate from midbrain neural crest cells, which do not express dlx genes, are less affected. Taken together with the observation that the normal patterns of visceral arch dlx expression just prior to cartilage condensation resemble the morphology of the cartilage elements that are about to differentiate, our results suggest that dlx genes are an important part of a multi-step process in the development of a subset of craniofacial cartilage elements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping