PUBLICATION
Structure and transcription of the gene for translation elongation factor 1 subunit alpha of zebrafish (Danio rerio)
- Authors
- Gao, D.Y., Li, Z.F., Murphy, T., and Sauerbier, W.
- ID
- ZDB-PUB-970320-1
- Date
- 1997
- Source
- Biochim. Biophys. Acta Gene Struct. Exp. 1350(1): 1-5 (Journal)
- Registered Authors
- Li, Zheng, Sauerbier, W.
- Keywords
- translation elongation factor 1a; gene structure; (zebrafish)
- MeSH Terms
-
- Zebrafish/genetics*
- Pseudogenes
- Gene Expression Regulation, Developmental
- Chromosome Mapping*
- Fertilization
- Haploidy
- Embryo, Nonmammalian/physiology
- Macromolecular Substances
- Transcription, Genetic*
- Restriction Mapping
- DNA Primers
- Peptide Elongation Factors/biosynthesis*
- Peptide Elongation Factors/genetics*
- Polymerase Chain Reaction
- Base Sequence
- Introns
- Genomic Library
- Peptide Elongation Factor 1
- Molecular Sequence Data
- Animals
- Exons
- PubMed
- 9003448 Full text @ Biochim. Biophys. Acta Gene Struct. Exp.
Citation
Gao, D.Y., Li, Z.F., Murphy, T., and Sauerbier, W. (1997) Structure and transcription of the gene for translation elongation factor 1 subunit alpha of zebrafish (Danio rerio). Biochim. Biophys. Acta Gene Struct. Exp.. 1350(1):1-5.
Abstract
The zebrafish gene for translation elongation factor 1 alpha (EF1 alpha) was isolated from a phage Lambda genomic library and sequence and structure determined. One gene copy of EF1 alpha per haploid set of chromosomes was found and no processed pseudogenes. A highly active promoter region was localized to a 277 bp PstI/PvuII fragment beginning 240 bp upstream from the tsp, but no transcription enhancing, or silencing activity was observed within 1 kbp upstream, or downstream from the promoter. Expression of EF1 alpha appears to be developmentally regulated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping