ZFIN ID: ZDB-PUB-970116-4 |
Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction
Fritz, A., Rozowski, M., Walker, C., and Westerfield, M.
Date: | 1996 |
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Source: | Genetics 144(4): 1735-1745 (Journal) |
Registered Authors: | Fritz, Andreas, Rozowski, Marion, Walker, Charline, Westerfield, Monte |
Keywords: | none |
MeSH Terms: |
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PubMed: | 8978059 |
Citation
Fritz, A., Rozowski, M., Walker, C., and Westerfield, M. (1996) Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction. Genetics. 144(4):1735-1745.
ABSTRACT
The ease with which mutations can be generated in zebrafish makes this vertebrate an important resource for developmental genetics and genome studies. We have developed a PCR-based screening method that allows the efficient identification of gamma-ray induced deficiencies targeted to selected sequences. We describe three mutants characteristic of our findings and show that these mutations include deletions and translocations that can affect as much as 1% of the genome. These deficiencies provide a basis for analyzing the functions of cloned zebrafish genes using noncomplementation screens for point mutations induced by high-efficiency chemical mutagenesis.
ADDITIONAL INFORMATION
- Genes / Markers (18)
- Phenotype Data
- Mutations and Transgenics (9)
- Fish (9)