ZFIN ID: ZDB-PUB-970102-15
Multiple elements may be used for regulation of the GAP-43 gene in different cell-types
Reinhard, E. and Skene, J.H.
Date: 1992
Source: Perspectives on developmental neurobiology   1: 29-37 (Journal)
Registered Authors: Reinhard, Eva, Skene, J.H.P.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Axons/metabolism
  • Dopa Decarboxylase/biosynthesis
  • Dopa Decarboxylase/genetics
  • Enhancer Elements, Genetic
  • Epidermis/embryology
  • GAP-43 Protein
  • Gastrula/physiology
  • Gene Expression Regulation*
  • Genes
  • Membrane Glycoproteins/genetics*
  • Nerve Tissue Proteins/genetics*
  • Nervous System/embryology
  • Organ Specificity
  • Promoter Regions, Genetic
  • Rats
  • Recombinant Fusion Proteins/biosynthesis
  • Regulatory Sequences, Nucleic Acid*
  • Signal Transduction
  • Species Specificity
  • Transcription Factors/metabolism
  • Zebrafish
PubMed: 1345682
Recent evidence suggests that GAP-43 expression is not restricted to the nervous system, but may also occur outside the neural cell lineage. Two distinct patterns of GAP-43 regulation can therefore be distinguished. The first is the regulation of GAP-43 expression in multiple cell-types, and the second is the gene's temporal modulation within one specific cell-type. The latter type is well documented for neurons, where GAP-43 regulation is regulated in a fashion that is dependent on axon integrity. Results from partial analysis of the GAP-43 promoter/enhancer region indicate that at least some of these aspects of GAP-43 gene regulation may be accounted for by distinct cis-acting elements. For example, the expression of a rat GAP-43 promoter fusion gene in epidermal cells of transgenic zebrafish is dependent on an enhancer element, that is clearly distinct from the minimal neural- specific promoter. Characterization of specific GAP-43 regulatory elements responsible for particular aspects of its regulation may provide insight to signal pathways also utilized by other genes during development. Ultimately, a better understanding of the molecular events during development could help to define more precisely the complex sequences necessary for the establishment of an intact organism.