PUBLICATION
Active transposition in zebrafish
- Authors
- Lam, W.L., Lee, T.S., and Gilbert, W.
- ID
- ZDB-PUB-961107-5
- Date
- 1996
- Source
- Proceedings of the National Academy of Sciences of the United States of America 93(20): 10870-10875 (Journal)
- Registered Authors
- Gilbert, Walter, Lam, Wan L.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Consensus Sequence
- DNA Transposable Elements*
- Electrophoresis, Gel, Two-Dimensional/methods
- Molecular Sequence Data
- Mutation*
- Pedigree
- Polymorphism, Genetic
- Sequence Alignment
- Zebrafish/genetics*
- PubMed
- 8855274 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Lam, W.L., Lee, T.S., and Gilbert, W. (1996) Active transposition in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 93(20):10870-10875.
Abstract
We have identified a new family of Tc1-like transposons in the zebrafish, Danio rerio. The sequence of a candidate active transposon, deduced from sample Tzf elements, shows limited resemblance to the previously described Tdr1 elements of zebrafish. Both the Tzf and the Tdr elements are extremely abundant in zebrafish. We describe here a general strategy for detecting transposition events in a complex genome and demonstrate its utility by selectively monitoring hundreds of potentially active Tzf copies in the zebrafish genome against a background of other related elements. We have followed members of a zebrafish pedigree, using this two-dimensional transposon display strategy, to identify the first examples of active transposition of such elements in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping