ZFIN ID: ZDB-PUB-961101-20
Zebrafish elav/huC homologue as a very early neuronal marker
Kim, C.H., Ueshima, E., Muraoka, O., Tanaka, H., Yeo, S.Y., Huh, T.L., and Miki, N.
Date: 1996
Source: Neuroscience letters   216(2): 109-112 (Journal)
Registered Authors: Huh, Tae-Lin, Kim, Cheol-Hee, Miki, Naomasa, Muraoka, Osamu, Tanaka, Hideomi, Yeo, Sang-Yeob
Keywords: zebrafish elav/HuC homologue; Hu proteins; neuron-specific RNA binding proteins; neuronal marker; neuronal determination; early neurogenesis
MeSH Terms:
  • Animals
  • Biomarkers
  • ELAV Proteins
  • Gene Expression Regulation, Developmental/physiology
  • In Situ Hybridization
  • Molecular Sequence Data
  • Neural Crest/chemistry
  • Neural Crest/cytology
  • Neurons/chemistry*
  • RNA, Messenger/analysis
  • Ribonucleoproteins/genetics*
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology*
PubMed: 8904795 Full text @ Neurosci. Lett.
Drosophila ELAV, a neuron-specific RNA binding protein, is expressed in all neurons right after their birth. This specific pattern of expression has led to its use as a pan-neuronal marker. At least three members of the elav family, HuD, HuC/ple21 and Hel-N1, have been reported to be neuron-specific in vertebrates, although it is unknown which member of this family is expressed at the time of early neuronal determination. We have isolated a zebrafish elav/HuC homologue (zHuC) which has 89% homology to human HuC protein. It is first expressed in the neuronal precursor cells in the neural plate immediately after gastrulation, and then high expression levels persist in most regions of the nervous system. HuC, like elav in Drosophila, may be one of the earliest neuronal markers in zebrafish.