PUBLICATION
Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord
- Authors
- Roelink, H., Augsburger, A., Heemskerk, J., Korzh, V., Norlin, S., Ruiz-i-Altaba, A., Tanabe, Y., Placzek, M., Edlund, T., Jessell, T.M., and Dodd, J.
- ID
- ZDB-PUB-961014-945
- Date
- 1994
- Source
- Cell 76: 761-775 (Journal)
- Registered Authors
- Korzh, Vladimir
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Differentiation
- Chick Embryo
- DNA Primers/chemistry
- Drosophila Proteins*
- Embryonic Induction*
- Extremities/embryology
- Gene Expression
- Hedgehog Proteins
- In Situ Hybridization
- Molecular Sequence Data
- Motor Neurons/cytology
- Nervous System/embryology*
- Proteins/chemistry
- Proteins/genetics*
- RNA, Messenger/genetics
- Rats
- Sequence Alignment
- Sequence Homology, Amino Acid
- Solubility
- Trans-Activators*
- Xenopus laevis
- Zebrafish/genetics
- Zebrafish Proteins
- PubMed
- 8124714 Full text @ Cell
Citation
Roelink, H., Augsburger, A., Heemskerk, J., Korzh, V., Norlin, S., Ruiz-i-Altaba, A., Tanabe, Y., Placzek, M., Edlund, T., Jessell, T.M., and Dodd, J. (1994) Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord. Cell. 76:761-775.
Abstract
The differentiation of distinct cell types in the ventral neural tube depends on local inductive signals from the notochord. We have isolated a vertebrate homolog of the Drosophila segment polarity gene hedgehog (hh) from zebrafish and rat, termed vhh-1. vhh-1 is expressed in the node, notochord, floor plate, and posterior limb bud mesenchyme. Each of these cell groups has floor plate inducing activity, suggesting that the vhh-1 gene may encode a floor plate-inducing molecule. Widespread expression of rat vhh-1 in frog embryos leads to ectopic floor plate differentiation in the neural tube. In vitro tests for the signaling functions of vhh-1 demonstrate that COS cells expressing the rat vhh-1 gene induce floor plate and motor neuron differentiation in neural plate explants. vhh-1 may, therefore, contribute to the floor plate and motor neuron inducing activities of the notochord.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping