PUBLICATION

Green fluorescent fusion proteins: powerful tools for monitoring protein expression in live zebrafish embryos

Authors
Peters, K.G., Rao, P.S., Bell, B.S., and Kindman, L.A.
ID
ZDB-PUB-961014-870
Date
1995
Source
Developmental Biology   171: 252-257 (Journal)
Registered Authors
Peters, Kevin G.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Glutathione Transferase/genetics
  • Glutathione Transferase/metabolism
  • Green Fluorescent Proteins
  • Luminescent Proteins/genetics*
  • Luminescent Proteins/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Transgenes
  • Xenopus
  • Zebrafish
PubMed
7556901 Full text @ Dev. Biol.
Citation
Peters, K.G., Rao, P.S., Bell, B.S., and Kindman, L.A. (1995) Green fluorescent fusion proteins: powerful tools for monitoring protein expression in live zebrafish embryos. Developmental Biology. 171:252-257.
Abstract
The recent development of transgenic technology in zebrafish has opened an exciting new avenue in which to explore vertebrate development. However, as in other species, the inability to easily identify live transgenic fish severely limits the potential of this promising technology. To determine whether the recently described green fluorescent protein (GFP) might provide a convenient live staining method in zebrafish, we constructed a glutathione S- transferase/GFP fusion protein (GST-GFP). GST-GFP cRNA, when injected into individual blastomeres of early zebrafish embryos, resulted in the rapid development (3 hr) of easily detectable green fluorescence which persisted for up to 4 days. GFP fluorescence was restricted to progeny of the injected cell and appeared to have no adverse effects on embryonic development despite widespread expression. Our findings demonstrate that GFP fusion proteins will provide a simple yet powerful means of monitoring production of heterologous proteins in live zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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