PUBLICATION
Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21
- Authors
- Njolstad, P.R., Molven, A., Hordvik, I., Apold, J., and Fjose, A.
- ID
- ZDB-PUB-961014-838
- Date
- 1988
- Source
- Nucleic acids research 16(19): 9097-9111 (Journal)
- Registered Authors
- Fjose, Anders, Molven, Anders
- Keywords
- none
- MeSH Terms
-
- Base Sequence
- Cloning, Molecular
- DNA/genetics
- Genes, Homeobox*
- Fishes/genetics*
- Animals
- Molecular Sequence Data
- Amino Acid Sequence
- RNA, Messenger/genetics
- Multigene Family
- Restriction Mapping
- Blotting, Northern
- Gene Expression Regulation
- PubMed
- 2902580 Full text @ Nucleic Acids Res.
Citation
Njolstad, P.R., Molven, A., Hordvik, I., Apold, J., and Fjose, A. (1988) Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21. Nucleic acids research. 16(19):9097-9111.
Abstract
We report the molecular cloning and characterization of a cDNA derived from a zebrafish gene (ZF-21) related to the mouse homeobox containing gene Hox2.1. Interesting information about the differential conservation of various domains was gained from comparisons between the putative protein sequences from ZF-21 (275 amino acids) and Hox2.1 (279 aa). A separate DNA binding domain including the ZF-21 homeodomain and 36 additional flanking residues is completely identical to the C- terminal part of Hox2.1. As a consequence, these two mouse and zebrafish proteins must have identical DNA binding properties. A lower level of sequence identity between the N- terminal coding regions of ZF-21 and Hox2.1 reduces the total protein homology to 81%. However, short stretches of perfect homology in these N-terminals suggests that the essential biochemical functions are the same. As expected for true homologues, the ZF-21 and Hox2.1 genes also share extensive similarities with respect to non-coding sequences and temporal expression during embryogenesis. The finding of a potential ZF- 21 duplication is discussed in relation to functional and evolutionary aspects of vertebrate homeobox genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping