PUBLICATION
Regulatory gene expression boundaries demarcate sites of neuronal differentiation in the embryonic zebrafish forebrain
- Authors
- Macdonald, R., Xu, Q., Barth, K.A., Mikkola, I., Holder, N., Fjose, A., Krauss, S., and Wilson, S.W.
- ID
- ZDB-PUB-961014-719
- Date
- 1994
- Source
- Neuron 13: 1039-1053 (Journal)
- Registered Authors
- Barth, Anukampa, Fjose, Anders, Holder, Nigel, Krauss, Stefan, Macdonald, Rachel, Wilson, Steve, Xu, Qiling
- Keywords
- none
- MeSH Terms
-
- Zebrafish
- Proto-Oncogene Proteins/genetics
- Repressor Proteins
- Wnt Proteins
- Homeodomain Proteins*
- Prosencephalon/embryology*
- Cell Differentiation
- Neural Pathways/embryology
- Forkhead Transcription Factors
- DNA-Binding Proteins/genetics
- Genes, Regulator
- Morphogenesis
- In Situ Hybridization
- Gene Expression Regulation, Developmental*
- Receptor Protein-Tyrosine Kinases/genetics
- Paired Box Transcription Factors
- PAX2 Transcription Factor
- Animals
- Transcription Factors/genetics
- Zebrafish Proteins*
- Neurons/cytology*
- Eye Proteins
- PubMed
- 7946344 Full text @ Neuron
Citation
Macdonald, R., Xu, Q., Barth, K.A., Mikkola, I., Holder, N., Fjose, A., Krauss, S., and Wilson, S.W. (1994) Regulatory gene expression boundaries demarcate sites of neuronal differentiation in the embryonic zebrafish forebrain. Neuron. 13:1039-1053.
Abstract
During development of the zebrafish forebrain, a simple scaffold of axon pathways is pioneered by a small number of neurons. We show that boundaries of expression domains of members of the eph, forkhead, pax, and wnt gene families correlate with the positions at which these neurons differentiate and extend axons. Analysis of genetically or experimentally altered forebrains indicates that if a boundary is maintained, there is appropriate neural differentiation with respect to the boundary. Conversely, in the absence of a boundary, there is concomitant disruption of neural patterning. We also show that a strip of cells within the dorsal diencephalon shares features with ventral midline cells. This strip of cells fails to develop in mutant fish in which specification of the ventral CNS is disrupted, suggesting that its development may be regulated by the same inductive pathways that pattern the ventral midline.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping