PUBLICATION

A mutation that changes cell movement and cell fate in the zebrafish embryo

Authors
Kimmel, C.B., Kane, D.A., Walker, C., Warga, R.M., and Rothman, M.B.
ID
ZDB-PUB-961014-583
Date
1989
Source
Nature   337: 358-362 (Journal)
Registered Authors
Kane, Donald A., Kimmel, Charles B., Walker, Charline, Warga, Rachel M.
Keywords
none
MeSH Terms
  • Animals
  • Cell Movement*
  • Crosses, Genetic
  • Cyprinidae/genetics*
  • Embryo, Nonmammalian/physiology*
  • Female
  • Heterozygote
  • Homozygote
  • Male
  • Morphogenesis
  • Mutation*
  • Phenotype
  • Zebrafish/genetics*
PubMed
2911386 Full text @ Nature
Abstract
The study of developmental patterning has been facilitated by the availability of mutations that produce changes in cell fate, in animals such as Caenorhabditis elegans and Drosophila melanogaster. We now describe a zygotic lethal mutation in the zebrafish, Brachydanio rerio, that also changes how particular embryonic cells develop. Severe pattern deficiencies are observed that are restricted to a single body region, the trunk. The mutation may directly affect mesoderm, as somites do not form in the trunk. Head and tail structures, including tail somites, are relatively undisturbed. The earliest detected expression of the mutation is during gastrulation, when movements of mesodermal cells occur incorrectly. We injected prospective trunk mesodermal cells with lineage tracer dye and observed that in mutants these cells may enter a new body region, the tail, and there may express a new fate appropriate for the changed position.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes