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ZIRC
ZFIN ID: ZDB-PUB-961014-565
The zebrafish Fgf-3 gene: cDNA sequence, transcript structure and genomic organization
Kiefer, P., Strähle, U., and Dickson, C.
Date: 1996
Source: Gene 168(2): 211-215 (Journal)
Registered Authors: Strähle, Uwe
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary
  • Fibroblast Growth Factor 3
  • Fibroblast Growth Factors/biosynthesis*
  • Fibroblast Growth Factors/genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • Protein-Tyrosine Kinases/biosynthesis
  • Protein-Tyrosine Kinases/genetics
  • Proto-Oncogene Proteins/biosynthesis*
  • Proto-Oncogene Proteins/genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic*
  • Xenopus Proteins*
  • Xenopus laevis
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 8654946 Full text @ Gene
ABSTRACT
We report the isolation and characterization of genomic and cDNA clones encoding zebrafish fibroblast growth factor 3 (FGF3). An initial cDNA clone was generated by PCR amplification using degenerate oligo primers corresponding to a conserved region of protein found in the mouse and human homologues. Screening a cDNA library made from 30-33-h-old zebrafish embryos with this PCR product led to the isolation of two cDNA clones. Sequence analysis of the longest cDNA insert (1810 bp) revealed a 256-amino-acid (aa) orf. The central region, composed of approx. 155 aa, shares 78% identity with the analogous region of Xenopus laevis FGF3 and 72% identity with the product of the more distantly related human gene. However, the N-and C-terminal domains of zebrafish FGF3 are very different from those of other known homologues. The cDNA was used as a probe on genomic DNA to create a physical map of the locus and to isolate a genomic clone encompassing the entire coding region and 5' sequences. DNA sequencing and RNase protection analyses indicate that zebrafish Fgf-3 (ZFgf-3) is structurally analogous to the mouse gene and regulated through two different promoters. The transcription start point of the proximal promoter aligns to that of mouse promoter P3 and lies within a conserved region of sequence.
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