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ZFIN ID: ZDB-PUB-961014-544
Zebrafish wnt8 and wnt8b share a common activity but are involved in distinct developmental pathways
Kelly, G.M., Greenstein, P., Erezyilmaz, D.F., and Moon, R.T.
Date: 1995
Source: Development (Cambridge, England)   121: 1787-1799 (Journal)
Registered Authors: Kelly, Greg, Moon, Randall T.
Keywords: Danio rerio, wnt, krox20, gsc, pax, snail1, spadetail, cyclops, mesoderm induction, pattern formation
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytoskeletal Proteins
  • DNA Primers
  • Embryonic Induction/genetics*
  • Female
  • Gene Expression
  • In Situ Hybridization
  • Mesoderm/physiology*
  • Molecular Sequence Data
  • Morphogenesis
  • Phenotype
  • Polymerase Chain Reaction
  • Proteins/genetics*
  • Sequence Alignment
  • Wnt Proteins
  • Xenopus
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 7600994
The specification of the vertebrate body plan is dependent on numerous signaling molecules, including members of the Wnt family. We have identified two zebrafish wnt8 paralogs related to Xwnt-8B and Xwnt-8, respectively. A RT-PCR assay demonstrated that wnt8 is expressed maternally, with transcripts detected throughout embryogenesis, whereas wnt8b transcripts were first detected during late gastrulation. The wnt8 transcripts at 50% epiboly are spatially restricted to those cells at the blastoderm margin, overlying gsc-expressing cells in the axial hypoblast. During late gastrulation, wnt8 was no longer detected in the marginal cells at the dorsal midline and by mid-segmentation, transcripts were found in the presumptive tail bud. In contrast, wnt8b expression is spatially restricted to prospective neuroepithelium, and later to neural-specific structures. Overexpression of both wnts results in two major phenotypes: radialized embryos and embryos with anterior defects. These phenotypes were preceded by significant changes in the spatial expression patterns of gsc and ntl transcripts, reminiscent of activities of Xwnt-8 in Xenopus, and consistent with a role for wnt8 in the specification or patterning of mesoderm.