PUBLICATION
Zebrafish pou[c]: a divergent POU family gene ubiquitously expressed during embryogenesis
- Authors
- Johansen, T., Moens, U., Holm, T., Fjose, A., and Krauss, S.
- ID
- ZDB-PUB-961014-492
- Date
- 1993
- Source
- Nucleic acids research 21: 475-483 (Journal)
- Registered Authors
- Fjose, Anders, Johansen, Terje, Krauss, Stefan
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Blotting, Northern
- DNA
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism
- Embryo, Nonmammalian/metabolism
- Humans
- Molecular Sequence Data
- Multigene Family*
- Nucleic Acid Conformation
- POU Domain Factors
- Sequence Homology, Amino Acid
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 8441661 Full text @ Nucleic Acids Res.
Citation
Johansen, T., Moens, U., Holm, T., Fjose, A., and Krauss, S. (1993) Zebrafish pou[c]: a divergent POU family gene ubiquitously expressed during embryogenesis. Nucleic acids research. 21:475-483.
Abstract
We report the isolation and characterization of cDNA for a novel zebrafish (Brachyodanio rerio) POU domain gene, pou[c], which is ubiquitously expressed during embryonic development. This gene encodes a 610 amino acids long protein with a 149 amino acid POU domain ending only 8 residues before the C terminus. The 453 amino acids long region N-terminal to the POU domain contains several features typical of transcriptional activation domains such as an acidic region with a putative amphipathic alpha-helix, a glutamine-rich region, and short threonine- and/or serine-rich regions. Comparison of the POU domain of pou[c] to other known POU sequences clearly show that pou[c] has the most divergent POU domain sequence reported to date. Thus, we suggest that pou[c] should be placed as the presently sole member of a new, sixth class of POU proteins. DNA-binding studies revealed that pou[c] is not an octamer-binding transcription factor like the Oct proteins described from mammals, chicken and Xenopus. Rather, pou[c] binds with high affinity to the TAATGARAT motif found in the promoters of the herpes simplex virus immediate early genes and to degenerate octamer-TAATGA motifs. Circular permutation analyses also show that pou[c] induces DNA bending upon sequence-specific binding.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping