Screen for mutations affecting development of zebrafish neural crest
- Henion, P.D., Raible, D.W., Beattie, C.E., Stoesser, K.L., Weston, J.A., and Eisen, J.S.
- Developmental genetics 18: 11-17 (Journal)
- Registered Authors
- Beattie, Christine, Eisen, Judith S., Henion, Paul, Raible, David, Weston, James A.
- MeSH Terms
- Crosses, Genetic
- Embryo, Nonmammalian/physiology
- Genetic Techniques
- Neural Crest/cytology
- Neural Crest/physiology*
- Point Mutation
- 8742830 Full text @ Dev. Genet.
Henion, P.D., Raible, D.W., Beattie, C.E., Stoesser, K.L., Weston, J.A., and Eisen, J.S. (1996) Screen for mutations affecting development of zebrafish neural crest. Developmental genetics. 18:11-17.
The neural crest provides a useful model to learn how cell fate diversification is regulated during vertebrate development. Our approach is to isolate zebrafish mutations in which the development of neural crest derivatives is disrupted, in order to learn about the underlying genetic mechanisms. We describe a screen in which parthenogenetic diploid embryos are examined both for visible phenotypes and for cellular defects in neural crest-derived sensory neurons recognized immunohistochemically. We present preliminary results from this screen and briefly describe a few representative mutations. We also discuss the general utility of our strategy and comment on the future directions of this approach.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes