PUBLICATION

Screen for mutations affecting development of zebrafish neural crest

Authors
Henion, P.D., Raible, D.W., Beattie, C.E., Stoesser, K.L., Weston, J.A., and Eisen, J.S.
ID
ZDB-PUB-961014-435
Date
1996
Source
Developmental genetics   18: 11-17 (Journal)
Registered Authors
Beattie, Christine, Eisen, Judith S., Henion, Paul, Raible, David, Weston, James A.
Keywords
none
MeSH Terms
  • Animals
  • Crosses, Genetic
  • Diploidy
  • Embryo, Nonmammalian/physiology
  • Female
  • Genetic Techniques
  • Male
  • Mutagenesis
  • Mutation*
  • Neural Crest/cytology
  • Neural Crest/physiology*
  • Parthenogenesis
  • Point Mutation
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
8742830 Full text @ Dev. Genet.
Abstract
The neural crest provides a useful model to learn how cell fate diversification is regulated during vertebrate development. Our approach is to isolate zebrafish mutations in which the development of neural crest derivatives is disrupted, in order to learn about the underlying genetic mechanisms. We describe a screen in which parthenogenetic diploid embryos are examined both for visible phenotypes and for cellular defects in neural crest-derived sensory neurons recognized immunohistochemically. We present preliminary results from this screen and briefly describe a few representative mutations. We also discuss the general utility of our strategy and comment on the future directions of this approach.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes