PUBLICATION

Mutations affecting skeletal muscle myofibril structure in the zebrafish

Authors
Felsenfeld, A.L., Walker, C., Westerfield, M., Kimmel, C., and Streisinger, G.
ID
ZDB-PUB-961014-300
Date
1990
Source
Development (Cambridge, England)   108: 443-459 (Journal)
Registered Authors
Felsenfeld, Adam, Kimmel, Charles B., Streisinger, George, Walker, Charline, Westerfield, Monte
Keywords
none
MeSH Terms
  • Animals
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophysiology
  • Female
  • Genes, Lethal/physiology*
  • Microscopy, Electron
  • Muscles/embryology*
  • Muscles/ultrastructure
  • Mutation*
  • Zebrafish
PubMed
2340809 Full text @ Development
Abstract
We describe embryonic lethal mutations in the zebrafish, Brachydanio rerio, which affect organization of skeletal muscle myofibrils. The mutations, fub-1(b45) and fub-1(b126), were independently isolated from progeny of gamma-irradiated females. Each segregates as a single recessive gene: b45 is located about 23 map units from its centromere. The b126 mutation has a similar but slightly larger apparent gene- centromere distance and a less severe phenotype. The two mutations fail to complement, suggesting that they are allelic. Homozygous b45 mutant embryos are paralyzed, and their axial skeletal muscle cells are unstriated, containing severely disorganized myofibrillar components. Gel-electrophoretic comparisons of b45 mutant and wild-type muscle proteins failed to reveal absent or altered major myofibrillar proteins. Embryos genetically mosaic for b45 were also phenotypically mosaic, suggesting that the defect is cell-autonomous. We suggest that these mutations identify a gene required for proper organization of skeletal muscle myofibrils, and that the more severe mutation may represent a null allele.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes