PUBLICATION
Active complex formation of type I and type II activin and TGF beta receptors in vivo as studied by overexpression in zebrafish embryos
- Authors
- DeVries, C.J.M., DeBoer, J., Joore, J., Strähle, U., VanAchterberg, T.A.E., Huylebröck, D., Verschüren, K., Miyazono, K., VandenEijnden-vanRaaij, A.J.M., and Zivkovic, D.
- ID
- ZDB-PUB-961014-226
- Date
- 1996
- Source
- Mechanisms of Development 54(2): 225-236 (Journal)
- Registered Authors
- Jongejan-Zivkovic, Dana, Joore, Jos, Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Activins
- Animals
- Base Sequence
- Inhibins/genetics
- Inhibins/metabolism*
- Mesoderm
- Mice
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Protein Binding
- Protein Serine-Threonine Kinases/metabolism
- RNA/administration & dosage
- Receptors, Transforming Growth Factor beta/genetics
- Receptors, Transforming Growth Factor beta/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 8652415 Full text @ Mech. Dev.
Citation
DeVries, C.J.M., DeBoer, J., Joore, J., Strähle, U., VanAchterberg, T.A.E., Huylebröck, D., Verschüren, K., Miyazono, K., VandenEijnden-vanRaaij, A.J.M., and Zivkovic, D. (1996) Active complex formation of type I and type II activin and TGF beta receptors in vivo as studied by overexpression in zebrafish embryos. Mechanisms of Development. 54(2):225-236.
Abstract
We have investigated the involvement of activin receptors and TGF beta type I receptor in zebrafish development. Overexpression of either full-length or a truncated form of mouse ActR-IIA interferes with the development. Different splice variants of mouse ActR-IIB have distinct effects; ActR- IIB4 induces abnormal embryos, whereas ActR-IIB2 does not. Activin and TGF beta type I receptors can induce axis duplications. Co-expression of ActR-IA or ActR-IB with the type II activin receptors results in a synergistic increase of the frequency of axis duplication. Moreover, ActR-IIB2 is synergistic with ActR-IA and ActR-IB, demonstrating that ActR- IIB2 can interact with the zebrafish ligand. Overexpression of TGF beta R-I with ActR-IIA or ActR IIB4 results in a synergistic increase in frequency of abnormal embryos, whereas in combination with ActR-IIB2 no such increase occurs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping