PUBLICATION
Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells [see comments]
- Authors
- Burns, J.C., Friedmann, T., Driever, W., Burrascano, M., and Yee, J.K.
- ID
- ZDB-PUB-961014-146
- Date
- 1993
- Source
- Proceedings of the National Academy of Sciences of the United States of America 90: 8033-8037 (Journal)
- Registered Authors
- Burns, Jane C., Driever, Wolfgang
- Keywords
- none
- MeSH Terms
-
- Helper Viruses/genetics
- Helper Viruses/metabolism
- Cell Line, Transformed
- Adenoviruses, Human/genetics*
- Genes, pol
- Viral Envelope Proteins/biosynthesis*
- Viral Envelope Proteins/genetics
- Animals
- Cricetinae
- Genetic Vectors
- Dogs
- Vesicular stomatitis Indiana virus/genetics*
- Vesicular stomatitis Indiana virus/metabolism
- Kidney
- Genes, gag
- Membrane Glycoproteins*
- Transfection
- Promoter Regions, Genetic
- Restriction Mapping
- Cell Line
- Moloney murine leukemia virus/genetics*
- Plasmids
- Cytomegalovirus/genetics
- Humans
- PubMed
- 8396259 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Burns, J.C., Friedmann, T., Driever, W., Burrascano, M., and Yee, J.K. (1993) Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells [see comments]. Proceedings of the National Academy of Sciences of the United States of America. 90:8033-8037.
Abstract
The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely replaced by the G glycoprotein of vesicular stomatitis virus. Such vectors can be concentrated by ultracentrifugation to titers > 10(9) colony-forming units/ml and can infect cells, such as hamster and fish cell lines, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein. The ability to concentrate vesicular stomatitis virus G glycoprotein pseudotyped vectors will facilitate gene therapy model studies and other gene transfer experiments that require direct delivery of vectors in vivo. The availability of these pseudotyped vectors will also facilitate genetic studies in nonmammalian species, including the important zebrafish developmental system, through the efficient introduction and expression of foreign genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping