PUBLICATION
Function of the Eph-related kinase rtk1 in patterning of the zebrafish forebrain
- Authors
- Xu, Q.L., Alldus, G., Macdonald, R., Wilkinson, D.G., and Holder, N.
- ID
- ZDB-PUB-961014-1283
- Date
- 1996
- Source
- Nature 381: 319-322 (Journal)
- Registered Authors
- Holder, Nigel, Macdonald, Rachel, Wilkinson, David, Xu, Qiling
- Keywords
- none
- MeSH Terms
-
- Animals
- Diencephalon/embryology
- Diencephalon/enzymology
- Eye/embryology
- Fetal Proteins/genetics
- Morphogenesis/physiology
- Prosencephalon/cytology
- Prosencephalon/embryology*
- Prosencephalon/enzymology
- RNA, Messenger/metabolism
- Receptor Protein-Tyrosine Kinases/genetics
- Receptor Protein-Tyrosine Kinases/physiology*
- Receptor, EphA4
- Zebrafish
- PubMed
- 8692269 Full text @ Nature
Citation
Xu, Q.L., Alldus, G., Macdonald, R., Wilkinson, D.G., and Holder, N. (1996) Function of the Eph-related kinase rtk1 in patterning of the zebrafish forebrain. Nature. 381:319-322.
Abstract
Early during its development, the vertebrate brain is subdivided into regions that have distinct fates and correlate with the expression domains of regulatory genes, but little is known about the cell-cell interactions that establish this spatial pattern. Candidates for regulating such interactions are the Eph-related receptor tyrosine kinases (RTKs) which have spatially restricted expression in the developing brain. These RTKs may mediate cell-contact-dependent signalling by interacting with membrane-bound ligands, and have been implicated in axon repulsion and the segmental restriction of gene expression in the hindbrain, but nothing is known regarding their function in the rostral neural epithelium. Here we use a dominant-negative approach in the zebrafish embryo to interfere with the function of Rtk1, an Eph-related RTK expressed in the developing diencephalon. We find that expression of a truncated receptor leads to expansion of the eye field into diencephalic territory and loss of diencephalic structures, indicating a role for Rtk1 in patterning the developing forebrain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping