PUBLICATION
Rank-dependent control of tuft and BEST4 cell development in the intestine
- Authors
- Willms, R.J., McCabe, T., Jones, L.O., Schade, R., Foley, E.
- ID
- ZDB-PUB-260520-20
- Date
- 2026
- Source
- Nature communications : (Journal)
- Registered Authors
- Foley, Edan
- Keywords
- none
- Datasets
- GEO:GSE303709
- MeSH Terms
- none
- PubMed
- 42156778 Full text @ Nat. Commun.
Citation
Willms, R.J., McCabe, T., Jones, L.O., Schade, R., Foley, E. (2026) Rank-dependent control of tuft and BEST4 cell development in the intestine. Nature communications. :.
Abstract
Specialist intestinal epithelial cells are critical for barrier integrity and immune responses at the mucosal boundary, yet the pathways that govern their development are incompletely defined. Here, we identify an essential role for TNFRSF11A/RANK in shaping intestinal epithelial specialization in zebrafish. Using lineage trajectory analysis, we identify two tuft cell subtypes, including a subtype enriched for expression of genes required to produce pro-inflammatory leukotrienes. We show that RANK deficiency reduces the abundance of immune-regulatory tuft and BEST4 cells, increases goblet cell frequency, and promotes the accumulation of pro-inflammatory leukocytes in the gut. Functionally, we demonstrate that the number of cells expressing the BEST4 cell marker cftr expand following infection with a pandemic strain of Vibrio cholerae, and we show that RANK deficiency enhances fish susceptibility to host colonization by Vibrio, implicating this lineage in host defenses against an enteric pathogen. Together, our findings implicate RANK signaling in intestinal epithelial diversification and immune regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping