PUBLICATION
ankrd1a is consistently upregulated in cardiomyocytes bordering the injury or scar area and affects their dedifferentiation during zebrafish heart regeneration
- Authors
- Boskovic, S., Novkovic, M., Milicevic, A., Milosevic, E., Jasnic, J., Milovanovic, M., Kojic, S.
- ID
- ZDB-PUB-260321-7
- Date
- 2026
- Source
- Cells & development : 204082 (Journal)
- Registered Authors
- Boskovic, Srdjan, Kojic, Snezana
- Keywords
- Border zone cardiomyocytes, Dedifferentiation, Heart regeneration, Zebrafish, ankrd1a
- MeSH Terms
- none
- PubMed
- 41861960 Full text @ Cells Dev
Citation
Boskovic, S., Novkovic, M., Milicevic, A., Milosevic, E., Jasnic, J., Milovanovic, M., Kojic, S. (2026) ankrd1a is consistently upregulated in cardiomyocytes bordering the injury or scar area and affects their dedifferentiation during zebrafish heart regeneration. Cells & development. :204082.
Abstract
In contrast to humans, zebrafish have an outstanding ability to regenerate injured heart through a highly orchestrated process involving all cardiac structures. To replace the lost myocardium, resident cardiomyocytes (CMs) dedifferentiate and proliferate, invading the injured area. The response of the myocardium is preceded by the activation of the epicardium and endocardium, which form active scaffolds to provide mechanical and paracrine support to guide regeneration. New CMs use protrusions to migrate and invade fibrotic injured tissue, replacing it with functional myocardium. Here, we investigated the expression profile of the stress-responsive ankrd1a gene in different cardiac structures, at key time points during regeneration, aiming to gain insight into its precise roles during zebrafish heart regeneration. In the TgBAC(ankrd1a:EGFP) reporter line, transgene upregulation was restricted to the myocardium, initiated as early as 15 h post-cryoinjury, and consistently marked CMs bordering the injury or scar area during regeneration. Transcriptome profiling and immunostaining revealed a potential role of ankrd1a in regulating CMs' dedifferentiation, as well as changes in the expression of genes associated with antigen presentation and extracellular matrix composition in the ankrd1a mutant. Our results indicate that ankrd1a is dispensable for ventricular regeneration after cryoinjury, while it may serve as a marker and fine-tuner in the healing process of injured cardiac muscle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping