PUBLICATION
Study of the Cytotoxic and Wound Healing Activity of Polysaccharides from Bovistella utriformis
- Authors
- Maaloul, A., Valverde-Guillén, P., Cárdenas-García, C., Pérez Manríquez, C., Araya-Rojas, M., Marí-Beffa, M., Fajardo, V., Abdala Díaz, R.T.
- ID
- ZDB-PUB-260227-43
- Date
- 2026
- Source
- Molecules 31: (Journal)
- Registered Authors
- Marí-Beffa, Manuel
- Keywords
- Bovistella utriformis, keratinocytes, polysaccharides, proteomics, wound healing, zebrafish regeneration
- MeSH Terms
-
- Animals
- Cell Cycle/drug effects
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Fungal Polysaccharides*/chemistry
- Fungal Polysaccharides*/pharmacology
- HaCaT Cells
- Humans
- Keratinocytes/drug effects
- Keratinocytes/metabolism
- Polysaccharides*/chemistry
- Polysaccharides*/pharmacology
- Proteomics
- Wound Healing*/drug effects
- Zebrafish
- PubMed
- 41752430 Full text @ Molecules
Citation
Maaloul, A., Valverde-Guillén, P., Cárdenas-García, C., Pérez Manríquez, C., Araya-Rojas, M., Marí-Beffa, M., Fajardo, V., Abdala Díaz, R.T. (2026) Study of the Cytotoxic and Wound Healing Activity of Polysaccharides from Bovistella utriformis. Molecules. 31:.
Abstract
Bovistella utriformis (Bull.) Demoulin & Rebriev is a cosmopolitan puffball mushroom traditionally recognized for its anti-inflammatory and wound-healing properties. This study aimed to evaluate the cytotoxic profile, regenerative potential, and molecular effects of polysaccharides extracted from B. utriformis (PsBU) using complementary in vitro and in vivo approaches. Thermogravimetric analysis revealed good thermal stability up to 300 °C with 26.91% residual mass at 800 °C. Human HaCaT keratinocytes were used to assess cell viability, proliferation, and cell-cycle distribution through MTT assays and flow cytometry, while wound-healing activity was examined using in vitro scratch assays. PsBU exhibited no cytotoxic effects across concentrations from 19.53 to 10,000 µg mL-1, with cell viability remaining above 68%. At 5000 and 10,000 µg mL-1, viability increased to 130% and 126%, respectively. The optimal in vitro wound-healing effect was observed at 500 µg mL-1, achieving 40% wound closure at 32 h. Label-free quantitative proteomic analysis by UHPLC-HRMS identified 83 differentially expressed proteins, including upregulation of tissue repair-related factors such as plasminogen and FHL2, alongside modulation of cell-cycle regulation and mRNA transport pathways. In vivo zebrafish caudal fin regeneration assays demonstrated maximal regenerative activity at 200 µg mL-1 (p < 0.001 vs. control). Overall, these findings demonstrate that B. utriformis polysaccharides are safe bioactive compounds that promote key biological processes involved in tissue regeneration, supporting their potential application as natural wound-healing agents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping