PUBLICATION
Bnip3lb-driven mitophagy maintains fate of the embryonic hematopoietic stem cell pool
- Authors
- Meader, E., Walcheck, M.T., Leder, M.R., Delaney, P., Falchetti, M., Jing, R., Li, C., Kambli, N.K., Wrighton, P.J., Sugden, W.W., Najia, M.A., Oderberg, I.M., Taylor, V.M., LeBlanc, Z.C., Quenzer, E.D., Lim, S.E., Schlaeger, T., Daley, G.Q., Goessling, W., North, T.E.
- ID
- ZDB-PUB-260225-11
- Date
- 2026
- Source
- Nature communications : (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 41735276 Full text @ Nat. Commun.
Citation
Meader, E., Walcheck, M.T., Leder, M.R., Delaney, P., Falchetti, M., Jing, R., Li, C., Kambli, N.K., Wrighton, P.J., Sugden, W.W., Najia, M.A., Oderberg, I.M., Taylor, V.M., LeBlanc, Z.C., Quenzer, E.D., Lim, S.E., Schlaeger, T., Daley, G.Q., Goessling, W., North, T.E. (2026) Bnip3lb-driven mitophagy maintains fate of the embryonic hematopoietic stem cell pool. Nature communications. :.
Abstract
Embryonic hematopoietic stem and progenitor cells (HSPCs) have the clinically valuable ability to undergo substantial proliferative expansion while maintaining multipotency, which remains difficult to replicate in culture. Here, we show that newly specified HSPCs achieve this unique state by precise spatio-temporal regulation of reactive oxygen species (ROS) via Bnip3lb-associated developmentally-programmed mitophagy, a distinct autophagic regulatory mechanism from that of adult HSPCs. While ROS drives HSPC specification in the dorsal aorta, scRNAseq and live-imaging of mitophagy-reporter zebrafish indicate that mitophagy initiates during endothelial-to-hematopoietic transition and colonization of secondary niches. Knockdown of bnip3lb reduces mitophagy and HSPC numbers in the caudal hematopoietic tissue by promoting myeloid-biased differentiation and apoptosis, which can be rescued by antioxidant exposure. Conversely, chemical or genetic induction of mitophagy enhances embryonic HSPC and lymphoid progenitor numbers. Significantly, compound-mediated mitophagy activation improves ex vivo function of HSPCs derived from human-induced pluripotent stem cells, enhancing serial-replating hematopoietic colony forming potential.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping